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October, 2011


© 2011, Hardy Diagnostics,

all rights reserved

Hardy offers…

SystemSure Plus

The most popular

ATP detection device
for sanitation monitoring.

The SystemSURE Plus ATP luminometer is the next generation of the best selling SystemSURE II.

Designed with state-of-the-art electronics and improved functionality this palm-sized system is easy to use, extremely sensitive and affordable.

Hygiena Ultrasnap

Used by large and small food processors, hospitals, restaurants, supermarkets, and other industries where rapid detection of contamination is critical.

The SystemSURE Plus allows companies to quickly determine the cleaning efficiency and hygienic status of surfaces and water, ensure product quality, and reduce costs.

Contact Hardy for all your surface monitoring needs;ATP,glucose,lactose, andproteinresidues.

See the catalog listing…

See the brochure on all ATP monitoring supplies….

I want more information and a price quote.

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Making life easier

for the researcher…

Do you do molecular biology research?

CulGenex Products

Let Hardy show you how to get instant delivery of your…

  • LB Agar
  • Gels
  • Agarose
  • Antibiotics
  • Buffers
  • Molecular grade water
  • Reagents
  • Chromogens
  • Transfection reagents
  • Competent cells

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Brainteasers

Brain


Who uttered these
“Famous Last Words”?

Enter here to take the test

Micro Tips…

Q: What is the best way to preserve my fungal cultures for long term storage?

A: The renowned mycologist, Jim Harris, PhD, recommends using sterile water for this purpose. Simply transfer the fungal elements (without media) with a sterile applicator stick to a tube of sterile water (cat no.K187). Cap tightly, and store at room temperature.

Most fungi will survive for decades with this method (however some zygomycetous fungi will only last months). Not only is this a good preservation method, but it reduces the chance of pleomorphism uponsubculture.

T. rubrum

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Phraseology

High on the Hog

Living “high on the hog” is a fairly recent expression originating in America in the 1920s. When a privileged or wealthy individual eats “high on the hog,” they are consuming the best cuts of meat from the pig, i.e. from the back or upper leg. Alternatively, the lower classes dine on the lower portions cut from the belly and trotters.

High on hog

~ ~ ~

The worst food-borne outbreak in 25 years

Cantaloupe

Find outabout what caused the recent outbreak that left 25 dead and 123 severely ill.

Think about it…

The Thinker

  • Can you cry under water?
  • How important does a person have to be before they are considered assassinated instead of just murdered?
  • Why do you have to “put your two cents in”… but it’s only a “penny for your thoughts?” Where does that extra penny go?
  • Once you’re in heaven, do you get stuck wearing the clothes you were buried in for eternity?

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Winning

Gandhi

First they ignore you.

Then they laugh at you.

Then they fight you.

Then you win.

Mohandas Gandhi

1869 ~ 1948

Leader of the independence movement in India.

Wisdom to ponder…

Steve Jobs 3

Steve Jobs

Co-founder and CEO

of Apple and Pixar.

Apple is now the most valuable company in the world.

1955 ~ 2011

“…A lot of times, people don’t know what they want until you show it to them.”

“Being the richest man in the cemetery doesn’t matter to me … Going to bed at night saying we’ve done something wonderful… that’s what matters to me.”

“I’m sorry, it’s true. Having children really changes your view on these things. We’re born, we live for a brief instant, and we die. It’s been happening for a long time. Technology is not changing it much – if at all.”

“…The most corrosive piece of technology that I’ve ever seen is called television – but then, again, television, at its best, is magnificent.”

“We made the buttons on the screen look so good you’ll want to lick them.”

“You know, my main reaction to this money thing is that it’s humorous, all the attention to it, because it’s hardly the most insightful or valuable thing that’s happened to me.”

“The only problem with Microsoft is they just have no taste. They have absolutely no taste. And I don’t mean that in a small way, I mean that in a big way, in the sense that they don’t think of original ideas, and they don’t bring much culture into their products.”

“I am saddened, not by Microsoft’s success – I have no problem with their success. They’ve earned their success, for the most part. I have a problem with the fact that they just make really third-rate products.”

“It’s more fun to be a pirate than to join the navy.”

“…And I remain extremely concerned when I see what’s happening in our country, which is in many ways the luckiest place in the world. We don’t seem to be excited about making our country a better place for our kids.”

“Your work is going to fill a large part of your life, and the only way to be truly satisfied is to do what you believe is great work. And the only way to do great work is to love what you do. If you haven’t found it yet, keep looking. Don’t settle. As with all matters of the heart, you’ll know when you find it. And, like any great relationship, it just gets better and better as the years roll on. So keep looking until you find it. Don’t settle.”

“For the past 33 years, I have looked in the mirror every morning and asked myself: “If today were the last day of my life, would I want to do what I am about to do today?” And whenever the answer has been “No” for too many days in a row, I know I need to change something…

Steve Jobs 2

“Remembering that I’ll be dead soon is the most important tool I’ve ever encountered to help me make the big choices in life. Because almost everything – all external expectations, all pride, all fear of embarrassment or failure – these things just fall away in the face of death, leaving only what is truly important. Remembering that you are going to die is the best way I know to avoid the trap of thinking you have something to lose. You are already naked. There is no reason not to follow your heart.”

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Did you know?
East Wing

Hardy Diagnostics…
  • Is celebrating its 31styear of serving microbiologists.
  • Manufactures over3,000 productsfor microbiology for you to choose from.
  • Maintains seven distribution centers in the U.S. for faster turn around time to your lab.
  • IsISO 13485certified for the manufacture of medical devices to give you confidence in our products.
  • Offers you its technical manual,HUGO, which contains over 4,500 pages of information regarding microbiology.
  • Maintains a worldwide network of over 30distributors.

Send amessage to the president.

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Micro Musings..

Cartoon Oct 2011

Case Study #4

Can You Identify

This Pathogen?

An 89 year old man presented to a community hospital emergency room with weakness and inability to walk.

Among other tests, two sets of blood cultures were drawn to rule out sepsis, and submitted to the Microbiology laboratory where they were put into an automated blood culture instrument for incubation.

Within twenty four hours both aerobic bottles and one anaerobic bottle flagged as positive. Aerobic bottles were subcultured to Sheep Blood, Chocolate and MacConkey agar plates incubated at 35-37C in 5-10% CO2, as well as an anaerobic Brucella agar plate incubated anaerobically. Anaerobic subculture included a BBE/LKV plate incubated anaerobically.

Direct and Day 1 gram stains showed small, non-spore forming gram positive rods.

Listeria gram stain

Gram stain of culture

Plates were examined on Day 1. A small, whitish, moist colony with a narrow zone of beta hemolysis around and under the colonies was noted on Blood agar plates.

Listeria plates

Left: blood agar plate; Right: same plate with transmitted light

Growth was noted on Chocolate and anaerobic Brucella agar plate. A Bile Esculin slant was inoculated and turned positive the next day.

A wet mount was done at the bench and the organism appeared very motile with a characteristic type of “tumbling” motility. The catalase test was positive. A CAMP test was performed; however, results were questionably negative.

An automated identification system identified the organism at the 99% level.

What is the organism?

See if you got it right…

molasses bottle
Anaerobic Bio-remediation

A “Sweet Solution” to Hazardous Waste

Recently, molasses is being used to clean up toxic waste and cancer-causing solvents. Large tanker trucks transport a diluted solution of molasses to the site of the contaminated soil. The solution is pumped into the ground through injection wells.

Molasses injection 2

There, it acts as a nutritious culture medium for naturally occurring microbes in the soil. Stimulated to multiply, the anaerobic bacteria break down toxic chemicals into the harmless byproducts: carbon dioxide, salt, and water.

These efforts have been known to decrease the concentration of toxic chemicals by up to 99%.  Sometimes cheese whey is added to the mix. Utilizing nature’s microbes is turning out to be a “sweet solution” to the growing problem of toxic waste on our planet.

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The Microbiology
Hall of Fame…

Emil Behring

Emil Adolf Behring is known as the great pioneer of serum therapy. His discovery of diphtheria and tetanus antitoxins paved the way for the prevention of disease through immunization.

Behring began his career in immunology as an assistant to Robert Koch at the Institute of Hygiene in Berlin. It was here that he joined a remarkable team of principal researchers investigating the new science of bacteriology.

While working at the Institute, Behring began research on diphtheria and tetanus. In the late 19th century, diphtheria was a leading cause of mortality in children. The disease, caused byCorynebacterium diphtheriae, can be traced back to the 4th to 5th century B.C. It was nicknamed the “strangling angel” because of the pseudomembrane that forms an adherent covering over the tonsils, pharynx and/or nasal cavity, obstructing breathing.

Likewise, tetanus is a predominately fatal conditioncharacterized by prolonged contraction of the skeletal muscle. Infection usually occurs through contamination of deep cuts or puncture wounds. The primary symptoms of tetanus are caused by a neurotoxin produced by the gram-positive, bacillus-shaped, obligate anaerobic bacterium Clostridium tetani.

Diphtheria toxin

Toxins produced byC. diphtheriaeandC. tetaniwere cultivated in 1888 and 1889, respectively, and, at the time, both organisms were known to cause disease when injected in pure form into healthy animals.

In 1890, Behring and a colleague on the Koch team, Shibasaburo Kitasato, published a joint paper famously entitled “The Mechanism of Immunity in Animals to Diphtheria and Tetanus.” The paper outlined how sterilized broth cultures of bacilli injected into the bodies of healthy animals resulted in the production of antitoxins capable of neutralizing these deadly toxins.

Within a week of this publication, Behring produced another article of a similar nature on immunity against diphtheria, outlining five ways in which it could be achieved. Behring and Kitasato later showed how serum obtained from animals exposed to toxins could be used to confer immunity to disease in other animals and, more astoundingly, even cure animals in the throes of deadly infection. The foundation of serum therapy and immunization by vaccination was dawning.

Behring in lab

Behring at right with lab partner and guinea pigs

Though Behring struggled to produce consistent results using diphtheria antitoxin in human subjects, Paul Ehrlich, another contemporary from the Institute of Hygiene, developed a process to standardize antitoxin production. Behring and Ehrlich went on to jointly develop a process to produce high-quality antitoxin from horses and sheep, paving the way for large-scale antitoxin production and widespread therapeutic use.

antitoxin

By 1895, Behring had been named professor and director of the Institute of Hygiene at the University of Marburg.

Behring continued to work with diphtheria during his directorship at Marburg and, in 1913, he announced the development of a novel toxin-antitoxin mixture used to provide longer-lasting immunity than serum-derived antitoxin. This approach to disease prevention, rather than treatment, became the forerunner for modern vaccine methods. To this day, children are routinely and effectively vaccinated against diphtheria and tetanus using vaccines based on Behring’s model.

Behring is credited for his contribution to the significant drop in diphtheria mortality around the end of the 19th century, and it is for this feat that he is primarily remembered. In 1901, Behring received the Nobel Prize for his work on serum therapy, “placing in the hands of the physician a victorious weapon against illness and death.”

With such notoriety, Behring was elevated to the status of nobility and received a sizeable cash prize. Other financial gains followed.  In 1914, he established a company to manufacture serums and vaccines; profits generated from this venture afforded him the fine luxuries of high society.

Behring logo

Despite outward appearances of considerable personal and professional accomplishments, Behring struggled with bouts of severe depression. In 1917, Behring contracted pneumonia and, soon thereafter, died of complications from the illness in Marburg, Germany. His work was held in such high regard, that foreign representatives of medical science from 26 nations attended his funeral services to pay tribute to him. His service was said to be a “deed of noble humanity” and his generosity towards science deserving of the “gratitude of the world.”

Antisera is also used for the identification of microorganisms. See our line ofDenke antisera.

Take our survey….

Mail delivery 2

Should the Post Office

Eliminate Saturday Delivery?

The mail volume in the U.S. has dropped by 30% from 2006 to 2010. Alarmingly, the post office lost more than $8 billion last year and is facing losses at least that large this year, despite having cut 110,000 jobs over the last four years and making other changes, including closing smaller, local post offices. Also, the post office wants to reduce mail delivery to five days-a-week, close an additional 3,700 offices and further cut the workforce by up to 220,000. The U.S. Postal Service is a self-funded government agency and receives no funding through taxes.



What did your fellow readers think?
See the results…

Should we increase oil exploration

and drilling in America?

Oil wells

At its peak in production, which occurred in
the 1970s, the U.S. produced about 10 million barrels of oil a day. Now, after 41 years of fairly steady decline, we produce about 5.5 million barrels a day, whereas we consume 20 million barrels daily.

Examine the survey results.

RUBES

Rubes Oct 2011

Find more

of Leigh Rubin’s cartoon humor.

Advice from the Founders…

Madison

“Philosophy is

common sense with big words.”

“If men were angels,
no government would be necessary.”
“All men having power
ought to be distrusted to a certain degree.”

*  *  *


James Madison

Fourth President of the United States

“Father of the U.S. Constitution”

1751 ~ 1836


Seeing is Bel
ieving?

Opt Ill Oct 2011
Art becomes the artist.

_______

“Believe half of what you see
and none of what you hear.”

~ Marvin Gaye

Key to success

The Secret of Success

Once a reporter interviewed a very successful leader…

Question:What’s the secret of your success?

Answer:Good decisions.

Question:How do you make so many good decisions?

Answer:Experience.

Question:How did you acquire so much experience?

Answer:Bad decisions.

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