Partnering with microbiologists to diagnose and prevent diseaseTM
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a culture of service…

January, 2013

© 2013, Hardy Diagnostics,

all rights reserved

To serve you better….

Distribution Centers

Distribution centers

Hardy now maintains eight distribution centers to get our products to you quickly and inexpensively.

Check them ou


  • Albany, New York
  • Lake City, Florida
  • Springboro, Ohio
  • Dallas, Texas
  • Centralia, Washington
  • Salt Lake City, Utah
  • Phoenix, Arizona
  • Santa Maria, California

The California and Ohio facilities also serve as ISO certified manufacturing centers where over 3,500 different microbiology products are made.

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What is a rapid and inexpensive way to identify

E. coli

from plated media?

Rapid indole is a good way to start, however, there are 52 species of gram-negative bacilli that grow on MacConkey, are indole-positive, and ferment lactose. The more common ones include

Klebsiella oxytoca


Citrobacter freundii, C. sedlakii, C. braakii, C. koserii, C. amalonaticus, C. youngae, Aeromonas hydrophila and Serratia odorifera.

Therefore, another rapid test is needed, such as glucuronidase. This test will give a positive result for most all

E. coli

strains, except

E. coli O157


Hardy ColiScreen combines both a rapid indole and glucuronidase test into one tube. Results are obtained from one to four hours.


ColiScreen tube showing a positive indole (red layer on top) and a positive glucuronidase test (yellow media) for

E. coli.


the instructions for use (IFU).


on order for ColiScreen.


a catalog of all rapid methods.

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Do you have a MicroTip or a case history to share with your fellow MicroBytes readers?

Click here.

Mini Catalogs

One-Stop Shopping

Sue catalog cover

Hardy stocks over 13,000 lab supply products that are available to all microbiologists at the click of a mouse!

No matter what kind of microbiology you do, we have a mini catalog suited to your needs.

Request a Mini Catalog that fits the needs of your laboratory . . .

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Check out


Brain Busters

for this month!

Stock up for 2013!



MBL Kwiksitks

Let Hardy Diagnostics be your one stop source for microorganisms.  Hardy is an authorized distributor for Microbiologics, where your organisms are sourced from ATCC, NCTC, or  NCIMB.


Advantages of KwikStiks:

1. Swab, hydrating fluid, and organism are provided in one foil pouch. No need for forceps, less chance of contamination.

2. Does not require frozen storage. Long shelf life.

3. Less cost! Compare our prices to the competition.

4. Traceable to reliable reference cultures. Certificates of Analysis available on-line.

KwikStik microorganisms are available in packages of 2 or 6.

Click here

to receive your catalog.

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Well Heeled

The term well heeled is used to refer to someone both well off financially or well dressed. Its origins actually lie in the blood-lust sport of cockfighting. If a bird was well heeled, it was in possession of good spurs – sharp and dangerous metal ‘heels’ attached to the back of a cock’s foot.

cock spurs

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Think about it…

The Thinker

* If all psychics know winning lottery numbers, why do they work?

* Why do we drive on parkways and park on driveways


* If you throw a cat out your car window does it become kitty litter?

* Why do they call them fingers? I’ve never seen them fing before


* Why isn’t 11 pronounced onety one?

* Why are aliens always green?

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Wisdom to ponder…

Frederick Douglas

Frederick Douglass

1818 ~ 1895

American orator, statesman, and author. After escaping from slavery, he became known as a leading champion of the movement for equal rights and the abolition of slavery.

“It is easier to build strong children than to repair broken men.”

“If there is no struggle, there is no progress.”

“The life of the nation is secure only while the nation is honest, truthful, and virtuous.”

“People might not get all they work for in this world, but they must certainly work for all they get.”

“I prayed for twenty years but received no answer until I prayed with my legs.”

“It is not light that we need, but fire; it is not the gentle shower, but thunder. We need the storm, the whirlwind, and the earthquake.”

“To suppress free speech is a double wrong. It violates the rights of the hearer as well as those of the speaker.”

“No man can put a chain about the ankle of his fellow man without at last finding the other end fastened about his own neck.”

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We crave your comments…


How are we doing?

Customer Feedback:

“I would like to commend Hardy’s employees for the quality service they have provided me. The shipments are prompt and when I talk to a representative I always am greeted pleasantly and helped immediately. Thank you for your high standards. You have an excellent staff. ”

Verla M. Andrews, Lab Technician

Barstow College

Are you a Hardy customer? If so, we need your help in assessing our performance.

At Hardy Diagnostics, we are fanatics about implementing Continuous Improvement. Would you please take a moment to answer two questions?

It should only take a minute or two and would be an immense help to us in honing our skills.

Provide some feedback…


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Want to receive the MicroBytes


at home?


Want to review past issues

of MicroBytes?

Did you know?

Val catalog cover

Hardy Diagnostics…
  • Is celebrating its 33rd year of serving microbiologists.
  • Is

    ISO 13485

    certified for the manufacture of medical devices to give you confidence in our products.
  • Offers you its technical manual,


    , which contains over 4,500 pages of information regarding microbiology.
  • Maintains a worldwide network of over 40


Send a

message to the president


Blue Logo

Micro Musings….

Cartoon Jan 2013

Case History #9

from Brent Barrett

A Puzzling Worm



hospital submitted a worm to the state public health laboratory for identification. It was removed during a colonoscopy from a 31 year old male. Further patient medical history was not provided.

Macroscopic observation showed a worm that measured about 20mm long and one end appeared to be broken off suggesting a longer overall length.

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CRKP’s increasing…



Klebsiella pneumoniae

. . . an increasing problem

A recent study tracked

Klebsiella pneumoniae

drug susceptibility over a 12 year period and found a significant increase in resistance to all drugs tested except for tetracycline.

Over 3.1 million susceptibility test reports from 1998 to 2010 were analyzed at the George Washington University in Washington DC.

Resistance to imipenem (a subgroup of carbapenem antibiotics) first appeared in the database in 2004 and rose gradually to 4.3% by the end of 2010.

The largest increases in antimicrobial drug resistance from 1998 to 2010 were observed for aztreonam (7.7% to 22.2%), ceftazidime (5.5% to 17.2%), and ciprofloxacin (5.5% to 16.8%). However, the researchers found smaller changes in resistance to tetracycline (14.2% to 16.7%) and amikacin (0.7% to 4.5%).

“Increasing antimicrobial drug resistance, including carbapenem-resistant

K. pneumoniae

(CRKP), accounts for substantial increases in illness and death and stresses health care resources,” the researchers write. “Few antimicrobial therapy options exist for infections caused by CRKP.”

In the current study, CRKP prevalence was higher for intensive care units than for other hospital units (6.3% vs 3.8%, respectively).

Newer-generation tetracyclines “may prove useful in the treatment of CRKP-related infections because of their improved tissue penetration, antimicrobial activity, and decreased propensity to develop antimicrobial drug resistance compared with their older counterparts,” the researchers write.

This emerging problem of increased resistance combined with a lack of novel antimicrobials in development present a major threat to public health and warrant due diligence in future surveillance efforts.

Hardy Diagnostics provides a

chromogenic culture media

for the initial screen of these organisms.

Learn more

from the CDC.

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Warning to green shoppers…

Reusable bags

Beware of the

Reusable Grocery Bag!

Recently, many municipalities have implemented bans or imposed taxes upon plastic or paper grocery bags on environmental grounds, in an effort to encourage shoppers to bring their own reusable bags.

San Francisco County was the first major U.S. jurisdiction to enact such a regulation, implementing a ban in 2007. There is evidence, however, that reusable grocery bags, a common substitute for plastic bags, contain potentially harmful bacteria.

A recent study at the University of Pennsylvania and George Mason University examined emergency room admissions related to these bacteria in the wake of the San Francisco ban. They found that ER visits spiked when the ban went into effect. Relative to other counties, ER admissions increase by at least one fourth, and deaths exhibit a similar increase.

“We were finding that deaths, for example, from foodborne illness or intestinal infections goes up from anywhere between 50% and 100%, so a doubling in some instances” according to the researcher, Jonathan Klick. He also says, “More people are showing up in the emergency rooms, and it turns out they have

E. coli


The danger arises when meat and produce in the resuable bags, that harbor pathogens like


and toxigenic strains of

E. coli

, are placed in a warm car during the ride home. Due to meat drippings and other residue, the pathogens remain viable for the next shopping trip.

Note to green shoppers….be sure to wash your grocery  bags!

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No coughing or sneezing required


Flu virus spreads
up to 6 feet

flu patient


he influenza virus can travel and be infectious within a six foot radius of a patient’s head according to a new study from the Wake Forest School of Medicine in North Carolina. Most alarming about this study is that the patients tested spread the virus even when not coughing or sneezing; they were simply breathing.

Investigators enrolled 94 patients with influenza-like illness in the study. They obtained patient history and took nasopharyngeal swab specimens. The researchers then collected quantitative impaction air samples 1 foot or less, 3 feet, and 6 feet from the patient’s head during routine care. Rapid test and polymerase chain reaction were used to detect influenza virus.

Also discovered was that some influenza patients (19%) are “super-emitters” shedding up to 32 times more viral particles than other patients. The size of the particle droplets were found to be much smaller at 6 feet than at 1 foot.

The N95 rated face masks

are effective in blocking 95% of particles greater than 0.3 microns in size, and can be useful in protecting health care workers as well as friends and family of the afflicted patients.

Learn more

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Optical oddities…

Opt Illusion jan 2013

Which face looks happier?

The right one? But how can that be when both faces are the same, except for being the mirror image of each other?

This is because the right hemisphere of our brains are more involved in the interpretation of facial expressions and em


“Believe half of what you see

and none of what you hear.”

~ Marvin Gaye

Get the survey results…

Should clinical labs

hire more MLT’s?

Lab Tech

Currently the management of clinical laboratories find that they must continually cut costs and increase efficiency.

Some labs do this by utilizing more and more the services of Medical Laboratory Technicians (MLT), rather than hiring the more highly paid and  extensively trained Clinical Laboratory Scientists (CLS). Is this a trend that should continue?

What did our readers say? …

Should medical device companies have to

pay a 2.3% excise tax?
Medical devices


tarting on January 1, 2013, all medical device manufacturers will be required to pay a 2.3% tax on all sales. The new tax, required by the Affordable Care Act (“Obamacare”), is levied on medical devices sold domestically.

Medical devices range from simple tongue depressors and bedpans to complex pacemakers and laser surgical devices. In addition, medical devices include

in vitro

diagnostic products, such as general purpose lab equipment, reagents, and test kits,

including culture media.

This tax is on all sales (top line) regardless of whether the company made a profit or not (bottom line). Medical device manufacturers are the only industry required to pay the 2.3% tax.

The tax is expected to bring in $20 billion to the federal government over the next 10 years. The House of Representatives voted to kill the tax, but the Senate is currently in favor of keeping it.

Will the new tax eliminate jobs, stifle innovation, and make American manufacturers less competitive?…or does the new tax represent a good way to help pay down the enormous federal deficit?

Are you in favor of keeping the tax?

See the survey results.

You may be shocked to find out the opinions were 81% to 19%!


Rubes Jan 2013

Find more

of Leigh Rubin’s cartoon humor.

WARNING: Never give chocolate to your dog. Not even on Valentine’s Day! The theobromine in chocolate can be lethal to dogs.

Learn more

Messy desk

A bus station is where a bus stops.

A train station is where a train stops.

On my desk, I have a work station…

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“If a cluttered desk is a sign of a cluttered mind, of what, then, is an empty desk a sign?”

~ Albert Einstein ~

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A clean desk is a sign of

a cluttered desk drawer.

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Pun Zone Ahead


  • The butcher backed into the meat grinder and got a little behind in his work.
  • I thought I saw an eye doctor on an Alaskan island, but it turned out to be an optical Aleutian.
  • She was only a whiskey maker, but he loved her still.
  • A rubber band pistol was confiscated from algebra class because it was a weapon of math disruption.
  • No matter how much you push the envelope, it’ll still be stationery.
  • The roundest knight at King Arthur’s round table

    was Sir Cumference. He

    acquired his size from too much pi.

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