Partnering with microbiologists to diagnose and prevent diseaseTM

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a culture of service…




December, 2014



© 2014, Hardy Diagnostics,

all rights reserved





Download Our
Mini Catalogs



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No matter what industry you may be in, Hardy has a mini catalog that contains the microbiology products that are needed in your lab.

  • Food and Beverage
  • Veterinary
  • Anaerobic Microbiology
  • Susceptibility Testing
  • Control Organisms
  • Cosmetics
  • Pharmaceuticals
  • Dehydrated Culture Media
  • And more…



View a complete list

and download the PDF version.



Request

the paper version.

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*  *  *







Brainteasers


Brain


Expose your mind to a high intensity workout!








Phraseology



“Play it again, Sam”

This phrase is the title of a Broadway play, a movie, a song, an instrumental piece, and numerous titles of commercial products.

It was a quote taken from the 1942 film “Casablanca” starring Humphrey Bogart. Oddly enough, Bogart never said “Play it again, Sam” in the film, he just said “Play it, Sam.”

This misquotation somehow stuck and is now more recognizable than what Bogart actually said.
* * *




Making life easier

for the microbiologist…



Rapid Test Kits




Hardy offers hundreds of rapid methods and time-saving short cuts. Find out how Hardy can help you with these “hard to find” products.


View

our on-line catalog.


Request

a paper catalog.


Ask


a question about our rapid methods.

Rapid MGP

*  *  *




CRITERION







W

hy should you be interested in Hardy’s CRITERION dehydrated culture media?
  • Wide mouth opening for ease of using a scoop. No shaking and dust formation needed.
  • All commonly used formulas are available in four different sizes, including a 2L pre-weighed mylar pouch.
  • Hardy ships anywhere in the world.
  • Competitively priced.
  • Quality is continually tested in Hardy’s 3,500 prepared media products.

Watch

a short video!

Contact us

for a price quote.

Request

a paper catalog.

See

the online catalog.

Learn

more…

*  *  *



Fast and easy…


StrepQuick

Hardy offers you a fast and easy way to identify Gram positive, catalase negative cocci.
StrepQuick

StrepQuick provides three rapid tests on one convenient card; LAP, ESC, and PYR.

StrepQuick is useful in the identification of:




Aerococcus viridans



Aerococcus urinae



Enterococcus spp.



Gemella hemolysans



Gemella morbillorum



Leuconostoc spp.



Pediococcus spp.



Streptococcus pyogenes (grp A)



Streptococcus agalactiae (grp B)



Streptococcus, group D



Streptococcus pneumoniae





Order

now.




See

the Instructions for use (IFU)

*  *  *




“Do or do not…

there is no trying.”
Yoda
* * *



How can we help?


That’s how many distribution centers Hardy Diagnostics maintains to serve you.

to watch a short video describing our distribution network that delivers fast and reliable service to your lab!

Click here

to find out which one services your home town.
____________________________

Recently, we added a “Chat” feature to our services. Now you can chat with us online or send in your questions by email, whichever suits you best.

Click here

to get some help right away!
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*  *  *






Think about it…




The Thinker


* Why do they put Braille on the drive-through bank machines?

* How do they get the deer to cross at that yellow road sign?

* Why do they sterilize the needles for lethal injections?

* Why did kamikaze pilots wear helmets?

* Is it true that cannibals don’t eat clowns because they taste funny?

* * *





Wisdom to ponder…




George Orwell

1903 ~ 1950


British journalist and novelist, who wrote about totalitarianism and social injustice. It was Orwell that coined such terms as

cold war

,

big brother

,

thought police

, and

thought crime

. His more famous works include

1984

and

Animal Farm.



“In a time of universal deceit – telling the truth is a revolutionary act.”



“At fifty everyone has the face he deserves.”



“Serious sport is war minus the shooting.”



“Freedom is the right to tell people what they do not want to hear”.


“Who controls the past controls the future. Who controls the present controls the past.”



“Early in life I had noticed that no event is ever correctly reported in a newspaper.”


“We sleep safe in our beds because rough men stand ready in the night to visit violence on those who would do us harm.”






“Each generation imagines itself to be more i


ntelligent than the one that went before it, and wiser than the one that comes after it.”



“Man is the only creature that consumes without producing. He does not give milk, he does not lay eggs, he is too weak to pull the plough, he cannot run fast enough to catch rabbits. Yet he is lord of all the animals.”

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QUICK LINKS…

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Want to receive the MicroBytes

Newsletter

at home?




CLICK HERE







Want to review past issues

of MicroBytes?











Did you know?

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Hardy Diagnostics…
  • Is celebrating its 35th year of serving microbiologists.
  • Is

    ISO 13485

    certified for the manufacture of medical devices to give you confidence in our products.
  • Offers you detailed technical inserts, comprised of over 4,500 pages of information regarding microbiology.
  • Services over 10,000 labs and maintains a worldwide network of over 65

    distributors.

  • Is an Employee-Owned company.

    “If we act like we own the place…it’s because we do!”


Send a


message to the president


.

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Micro Musings…










How is the

micro-community coping with drought and climate change?



O

ur planet is constantly changing. How are the microorganisms handling this change?




Crab blood used to test drugs



Endotoxin Detection


If you have ever had an injection before, chances are that injectable drug or vaccine was tested using the blood of the horseshoe crab shown above.

Bacterial endotoxins, also known as lipopolysacharides (LPS), are molecules that make up an important part of the gram negative bacterial cell wall. These molecules are responsible for structural integrity of the cell wall, protection from certain kinds of chemical attack, and maintenance of the cell membrane negative charge.

LPS are also an important virulence factor, and are secreted by gram negative bacteria through the membrane vesicle trafficking system, which releases these molecules, other proteins and virulence factors into the environment.

When endotoxin is released into the human blood stream, the LPS bind the CD14 receptor complex on many immune cells, including monocytes, macrophages, and B cells. Upon binding, these cells secrete pro-inflammatory cytokines, nitric oxide, and other immune response molecules.

This causes the body to go into septic shock, characterized by a drop in blood pressure, fever, and widespread blood clotting which can often result in death.

Thus, testing for the presence of endotoxin is of prime importance for pharmaceuticals and compounded sterile preparations. Any injectible drug that contains endotoxin could pose a dangerous threat to patient health.

Three types of endotoxin tests exist: the chromogenic method, the turbidimetric method, and the gel clot method. The gel clot method is considered the gold standard and believed to have the most accuracy and sensitivity.

The gel clot method uses blood from the Horseshoe crab (

Limulus polyphenus

). Unlike vertebrates, horseshoe crabs do not have

hemoglobin

in their blood, but instead use

hemocyanin

to carry oxygen. Because of the copper present in hemocyanin, their blood is blue. This blue blood contains amebocytes similar to white blood cells of vertebrates in defending the organism against pathogens.

These blood cells are used to make

Limulus Amebocyte Lysate (LAL)

, which is used for the detection of bacterial endotoxins in medical applications.  The blood of horseshoe crabs is harvested for this purpose.

H

arvesting horseshoe crab blood involves collecting and bleeding the animals, and then releasing them back into the sea.


Horseshoe crabs donating their blue blood to create LAL for endotoxin testing.
When the enzymes from the amebocytes are exposed to endotoxins, they form a clot or gel.

Hardy Diagnostics now offers Associates of Cape Cod, Inc., Pyrosate Test Kits. These kits test for endotoxin presence using the Limulus Amaebocyte Lysate (LAL) gel clot method. When used according to the Limit Test and Quantitative Test procedures, these kits are fully compatible with United States Pharmacopeia (USP) Bacterial Endotoxin Test (BET) guidelines. Kits are sold in three sensitivity levels, 0.03 EU/mL, 0.125 EU/mL, and 0.25 EU/mL. Each sensitivity level is available in kits of 10 or 30 tests. Endotoxin-free transfer pipettes and reagent grade water are also available for use with these kits, if necessary.



The Pyrosate Test Kit


is easy to use, rapid, and specific for endotoxin. Results are read simply by inverting the sample test tube. If a gel clot forms, the test is positive for endotoxin at or above the listed concentration.  Please refer to the product’s Instructions for Use (IFU) for a more detailed description of the procedure and analysis of results.

To see the complete line of products, click

HERE

. If you have any questions regarding this new product line, please

contact your Hardy Diagnostics Sales Representative

or call Technical Services at 1-800-266-2222, option 2.


by Raquel Kahler




Optical oddities…




What is it?


Scroll to the bottom of this column for answer.



“Believe half of what you see


and none of what you hear.”

~ Marvin Gaye ~





RUBES





Find more

of Leigh Rubin’s cartoon humor.

Want to

book Leigh

as a speaker at your next event?




Just the way you want it…


SpeedStreaks

Speedstreaks

Disposable sterile loops make your day go faster! Try out Hardy’s complete line of SpeedStreaks and see for yourself. They are available in the 1 or 10ul size; also available in flexible or rigid plastic.

Request

a sample of SpeedStreaks.

More information

on all types of loops.

Order

some SpeedStreaks.

*  *  *





78%

of young people say that they have

read a text message while driving.


Distracted driving is a dangerous


epidemic on America’s roadways.



In 2012 alone, 3,328 Americans


were killed in


distracted driving crashes. That’s about 1,000 more than


13 years of war in


Afghanistan


.





















Deck the halls with





boughs of fungi…





For those mycologists that just have to know what’s in our Petri plate tree…


Tree:

Aspergillus nidulans



Ornaments:

Penicillium marneffei





Trunk:

Aspergillus terreus.





Top:

Talaromyces stipitatus






From the J. Craig Ventner Blog.






“We need men that can


dream


of things that never were


and ask why not.”





John F. Kennedy




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Online Ordering Made Easy!

Online ordering


Watch

a short video

to learn how easy it is

to order from Hardy on-line!


Pick

. Click. You’re Done!



* * *


Our Optical Oddity for

the month (pictured above) is


a photo of a cross section of a communications cable that is laid at the bottom of the ocean.
The first sub-marine cable was laid in the 1850s to link telegraph stations. Today, cables link all the continents of the world except for Antarctica. The first fiber optic cable was laid in 1988.