Partnering with microbiologists to diagnose and prevent diseaseTM

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a culture of service…

December, 2014

© 2014, Hardy Diagnostics,

all rights reserved

Download Our
Mini Catalogs

Sue catalog cover

No matter what industry you may be in, Hardy has a mini catalog that contains the microbiology products that are needed in your lab.

  • Food and Beverage
  • Veterinary
  • Anaerobic Microbiology
  • Susceptibility Testing
  • Control Organisms
  • Cosmetics
  • Pharmaceuticals
  • Dehydrated Culture Media
  • And more…

View a complete list

and download the PDF version.


the paper version.

Val catalog cover

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Expose your mind to a high intensity workout!


“Play it again, Sam”

This phrase is the title of a Broadway play, a movie, a song, an instrumental piece, and numerous titles of commercial products.

It was a quote taken from the 1942 film “Casablanca” starring Humphrey Bogart. Oddly enough, Bogart never said “Play it again, Sam” in the film, he just said “Play it, Sam.”

This misquotation somehow stuck and is now more recognizable than what Bogart actually said.
* * *

Making life easier

for the microbiologist…

Rapid Test Kits

Hardy offers hundreds of rapid methods and time-saving short cuts. Find out how Hardy can help you with these “hard to find” products.


our on-line catalog.


a paper catalog.


a question about our rapid methods.

Rapid MGP

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hy should you be interested in Hardy’s CRITERION dehydrated culture media?
  • Wide mouth opening for ease of using a scoop. No shaking and dust formation needed.
  • All commonly used formulas are available in four different sizes, including a 2L pre-weighed mylar pouch.
  • Hardy ships anywhere in the world.
  • Competitively priced.
  • Quality is continually tested in Hardy’s 3,500 prepared media products.


a short video!

Contact us

for a price quote.


a paper catalog.


the online catalog.



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Fast and easy…


Hardy offers you a fast and easy way to identify Gram positive, catalase negative cocci.

StrepQuick provides three rapid tests on one convenient card; LAP, ESC, and PYR.

StrepQuick is useful in the identification of:

Aerococcus viridans

Aerococcus urinae

Enterococcus spp.

Gemella hemolysans

Gemella morbillorum

Leuconostoc spp.

Pediococcus spp.

Streptococcus pyogenes (grp A)

Streptococcus agalactiae (grp B)

Streptococcus, group D

Streptococcus pneumoniae




the Instructions for use (IFU)

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“Do or do not…

there is no trying.”
* * *

How can we help?

That’s how many distribution centers Hardy Diagnostics maintains to serve you.

to watch a short video describing our distribution network that delivers fast and reliable service to your lab!

Click here

to find out which one services your home town.

Recently, we added a “Chat” feature to our services. Now you can chat with us online or send in your questions by email, whichever suits you best.

Click here

to get some help right away!
Blue Logo

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Think about it…

The Thinker

* Why do they put Braille on the drive-through bank machines?

* How do they get the deer to cross at that yellow road sign?

* Why do they sterilize the needles for lethal injections?

* Why did kamikaze pilots wear helmets?

* Is it true that cannibals don’t eat clowns because they taste funny?

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Wisdom to ponder…

George Orwell

1903 ~ 1950

British journalist and novelist, who wrote about totalitarianism and social injustice. It was Orwell that coined such terms as

cold war


big brother


thought police

, and

thought crime

. His more famous works include



Animal Farm.

“In a time of universal deceit – telling the truth is a revolutionary act.”

“At fifty everyone has the face he deserves.”

“Serious sport is war minus the shooting.”

“Freedom is the right to tell people what they do not want to hear”.

“Who controls the past controls the future. Who controls the present controls the past.”

“Early in life I had noticed that no event is ever correctly reported in a newspaper.”

“We sleep safe in our beds because rough men stand ready in the night to visit violence on those who would do us harm.”

“Each generation imagines itself to be more i

ntelligent than the one that went before it, and wiser than the one that comes after it.”

“Man is the only creature that consumes without producing. He does not give milk, he does not lay eggs, he is too weak to pull the plough, he cannot run fast enough to catch rabbits. Yet he is lord of all the animals.”

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Want to receive the MicroBytes


at home?


Want to review past issues

of MicroBytes?

Did you know?

Val catalog cover

Hardy Diagnostics…
  • Is celebrating its 35th year of serving microbiologists.
  • Is

    ISO 13485

    certified for the manufacture of medical devices to give you confidence in our products.
  • Offers you detailed technical inserts, comprised of over 4,500 pages of information regarding microbiology.
  • Services over 10,000 labs and maintains a worldwide network of over 65


  • Is an Employee-Owned company.

    “If we act like we own the place…it’s because we do!”

Send a

message to the president


Blue Logo

Micro Musings…

How is the

micro-community coping with drought and climate change?


ur planet is constantly changing. How are the microorganisms handling this change?

Crab blood used to test drugs

Endotoxin Detection

If you have ever had an injection before, chances are that injectable drug or vaccine was tested using the blood of the horseshoe crab shown above.

Bacterial endotoxins, also known as lipopolysacharides (LPS), are molecules that make up an important part of the gram negative bacterial cell wall. These molecules are responsible for structural integrity of the cell wall, protection from certain kinds of chemical attack, and maintenance of the cell membrane negative charge.

LPS are also an important virulence factor, and are secreted by gram negative bacteria through the membrane vesicle trafficking system, which releases these molecules, other proteins and virulence factors into the environment.

When endotoxin is released into the human blood stream, the LPS bind the CD14 receptor complex on many immune cells, including monocytes, macrophages, and B cells. Upon binding, these cells secrete pro-inflammatory cytokines, nitric oxide, and other immune response molecules.

This causes the body to go into septic shock, characterized by a drop in blood pressure, fever, and widespread blood clotting which can often result in death.

Thus, testing for the presence of endotoxin is of prime importance for pharmaceuticals and compounded sterile preparations. Any injectible drug that contains endotoxin could pose a dangerous threat to patient health.

Three types of endotoxin tests exist: the chromogenic method, the turbidimetric method, and the gel clot method. The gel clot method is considered the gold standard and believed to have the most accuracy and sensitivity.

The gel clot method uses blood from the Horseshoe crab (

Limulus polyphenus

). Unlike vertebrates, horseshoe crabs do not have


in their blood, but instead use


to carry oxygen. Because of the copper present in hemocyanin, their blood is blue. This blue blood contains amebocytes similar to white blood cells of vertebrates in defending the organism against pathogens.

These blood cells are used to make

Limulus Amebocyte Lysate (LAL)

, which is used for the detection of bacterial endotoxins in medical applications.  The blood of horseshoe crabs is harvested for this purpose.


arvesting horseshoe crab blood involves collecting and bleeding the animals, and then releasing them back into the sea.

Horseshoe crabs donating their blue blood to create LAL for endotoxin testing.
When the enzymes from the amebocytes are exposed to endotoxins, they form a clot or gel.

Hardy Diagnostics now offers Associates of Cape Cod, Inc., Pyrosate Test Kits. These kits test for endotoxin presence using the Limulus Amaebocyte Lysate (LAL) gel clot method. When used according to the Limit Test and Quantitative Test procedures, these kits are fully compatible with United States Pharmacopeia (USP) Bacterial Endotoxin Test (BET) guidelines. Kits are sold in three sensitivity levels, 0.03 EU/mL, 0.125 EU/mL, and 0.25 EU/mL. Each sensitivity level is available in kits of 10 or 30 tests. Endotoxin-free transfer pipettes and reagent grade water are also available for use with these kits, if necessary.

The Pyrosate Test Kit

is easy to use, rapid, and specific for endotoxin. Results are read simply by inverting the sample test tube. If a gel clot forms, the test is positive for endotoxin at or above the listed concentration.  Please refer to the product’s Instructions for Use (IFU) for a more detailed description of the procedure and analysis of results.

To see the complete line of products, click


. If you have any questions regarding this new product line, please

contact your Hardy Diagnostics Sales Representative

or call Technical Services at 1-800-266-2222, option 2.

by Raquel Kahler

Optical oddities…

What is it?

Scroll to the bottom of this column for answer.

“Believe half of what you see

and none of what you hear.”

~ Marvin Gaye ~


Find more

of Leigh Rubin’s cartoon humor.

Want to

book Leigh

as a speaker at your next event?

Just the way you want it…



Disposable sterile loops make your day go faster! Try out Hardy’s complete line of SpeedStreaks and see for yourself. They are available in the 1 or 10ul size; also available in flexible or rigid plastic.


a sample of SpeedStreaks.

More information

on all types of loops.


some SpeedStreaks.

*  *  *


of young people say that they have

read a text message while driving.

Distracted driving is a dangerous

epidemic on America’s roadways.

In 2012 alone, 3,328 Americans

were killed in

distracted driving crashes. That’s about 1,000 more than

13 years of war in



Deck the halls with

boughs of fungi…

For those mycologists that just have to know what’s in our Petri plate tree…


Aspergillus nidulans


Penicillium marneffei


Aspergillus terreus.


Talaromyces stipitatus

From the J. Craig Ventner Blog.

“We need men that can


of things that never were

and ask why not.”

John F. Kennedy

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Online Ordering Made Easy!

Online ordering


a short video

to learn how easy it is

to order from Hardy on-line!


. Click. You’re Done!

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Our Optical Oddity for

the month (pictured above) is

a photo of a cross section of a communications cable that is laid at the bottom of the ocean.
The first sub-marine cable was laid in the 1850s to link telegraph stations. Today, cables link all the continents of the world except for Antarctica. The first fiber optic cable was laid in 1988.