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a culture of service…

March, 2015

© 2015, Hardy Diagnostics,

all rights reserved



Hardy offers a complete line of antibiotic disks that are fully compatible with your BD disk dispenser. The cartridges now feature “last disk recognition” so that you will know when its time for a refill.

See our complete catalog

of susceptibility testing products.


a price quote.
MH plate

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Save time…save money!



Now there’s a rapid and inexpensive way to help ID your gram positive cocci. StrepQuick performs three rapid tests within only 10 minutes; PYR, LAP, and Esculin.
StrepQuick is useful for the rapid ID of:

  • Aerococcus viridans

  • Aerococcus urinae

  • Enterococcus spp.

  • Gemella hemolysans

  • Gemella morbillorum

  • Leuconostoc spp.

  • Pediococcus spp.

  • S. pyogenes (group A)

  • S. agalactiae (group B)

  • Streptococcus, group D

  • Streptococcus pneumoniae


the Instructions (IFU)


the CLSI Guide to Rapid Testing.


our complete rapid test catalog.


the StrepQuick now.

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Animal Blood Products

Whatever your need is for animal blood products, Hardy has it. Our fresh weekly shipments come from ranches located in Oregon and Washington. All donor animals are humanely treated and are on a veterinary supervised program.

See our complete catalog

of blood products.


a price quote.

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Download Our
Mini Catalogs

Sue catalog cover

No matter what industry you may be in, Hardy has a mini catalog that contains the microbiology products that are needed in your lab.

  • Food and Beverage
  • Veterinary
  • Anaerobic Microbiology
  • Susceptibility Testing
  • Control Organisms
  • Cosmetics
  • Pharmaceuticals
  • Dehydrated Culture Media
  • And more…

View a complete list

and download the PDF version.


the paper version.

Val catalog cover

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Experience a cranial
melt down with
this month’s teasers.


Getting off

“Scot Free”

You might think this has something to do with people from Scotland.

Actually the phrase “scot-free” comes from Scandinavia and the Viking invasions of the British Isles. The invaders word for taxes was “sceot” or “scot.” To be declared “scot-free” meant that you had pleased your master and no longer owed a tax.
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New Initiative


The Obama administration recently released a new program to help stem the spread of highly resistant microorganisms. The title of this initiative is “National Action Plan for Combating Antibiotic-Resistant Bacteria.”
The five goals named in this new plan are as follows:

1. Slow the Emergence of Resistant Bacteria and Prevent the Spread of Resistant Infections.

2. Strengthen National One-Health Surveillance Efforts to Combat Resistance.

3. Advance Development and Use of Rapid and Innovative Diagnostic Tests for Identification and

Characterization of Resistant Bacteria.

4. Accelerate Basic and Applied Research and Development for New Antibiotics, Other

Therapeutics, and Vaccines.

5. Improve International Collaboration and Capacities for Antibiotic-resistance Prevention,

Surveillance, Control, and Antibiotic Research and Development.

One of the objectives is to increase the capability of laboratories to correctly identify the following organisms and their drug resistance status as named by the WHO:


Escherichia coli

: resistance to 3rd generation cephalosporins and to fluoroquinolones


Klebsiella pneumoniae

: resistance to 3rd generation cephalosporins and to carbapenems


Staphylococcus aureus

: methicillin resistance, or MRSA


Streptococcus pneumoniae

: resistance (non-susceptibility) to penicillin

* Non-typhoidal


(NTS): resistance to fluoroquinolones



species: resistance to fluoroquinolones


Neisseria gonorrhoeae

: reduced susceptibility to 3rd generation cephalosporins

See the complete text of the action plan.

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Think about it…

The Thinker

* Why isn’t there mouse-flavored cat food?

* What do you do when you see an endangered animal eating an endangered plant?

* If a parsley farmer is sued, can they garnish his wages?

* Would a fly without wings be called a walk?

* Why do they lock gas station bathrooms? Are they afraid someone will clean them?

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Wisdom to ponder…



1840 ~ 1904

Native American chief of the Nez Perce tribe in Oregon. His tribe was forced to relocate, then was pursued for 1,170 miles to the north by the US Army in 1877.

“You might as well expect rivers to run backwards as any man born free to be contented penned up.”

“The earth is the mother of all people, and all people should have equal rights upon it.”

“It makes my heart sick when I remember all the good words and the broken promises.”

“I believe much trouble would be saved if we opened our hearts more.”

“It does not require many words to speak the truth.”

“Treat all men alike. Give them the same law. Give them an even chance to live and grow.”

“All men were made by the Great Spirit Chief. They are all brothers.”

Hear me, my chiefs! I am tired. My heart is sick and sad. From where the sun now stands, I will fight no more forever.”

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Want to receive the MicroBytes


at home?


Want to review past issues

of MicroBytes?

Did you know?


Hardy Diagnostics…
  • Is celebrating its 35th year of serving microbiologists.
  • Is

    ISO 13485

    certified for the manufacture of medical devices to give you confidence in our products.
  • Offers you detailed technical inserts, comprised of over 4,500 pages of information regarding microbiology.
  • Services over 10,000 labs and maintains a worldwide network of over 65


  • Is an Employee-Owned company.

    “If we act like we own the place…it’s because we do!”

Send a

message to the president


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Micro Musings…

Murphy, don’t you think you’re getting
a little too attached to the lab animals?

CRE outbreak and

duodenoscope sampling


Found in Scopes


n the past few years there have been multiple reports of patient exposure to multi-drug resistant organisms, such as carbapenem-resistant Enterobacteriaceae (CRE), from contaminated duodenoscopes.

The tip of the duodenoscope has crevices that could harbor pathogenic bacteria.

In 2013, Advocate Lutheran General Hospital in Park Ridge, Illinois confirmed 38 cases of CRE transmission (you can read more about this case in our

February 2014 MicroBytes


Last month, Ronald Reagan UCLA Medical Center in Los Angeles notified 179 patients of possible exposure and has identified seven patients with infections. Sadly, the infections were contributing factors to the death of two patients.

More recently, Cedars-Sinai in Los Angeles notified 71 patients of possible exposure and has identified four patients with CRE transmission to date.

The transmission of CRE occurred from contaminated duodenoscopes used to perform endoscopic retrograde cholangiopancreatography (ERCP) procedures.

An ERCP is a test that combines the

use of a flexible, lighted scope (


) with X-rays to examine the ducts that drain the liver, gallbladder, and pancreas. Over 500,000 of these procedures are performed each year, resulting in the need to reprocess each duodenoscope multiple times a year.

The complex design of the duodenoscope results in difficult to access parts and hard to clean areas. Debris from previous patients may not be completely removed during cleaning, which allows for organisms to survive the disinfection process. As demonstrated by the recent outbreaks, multi-drug resistant organisms can be transmitted from patient to patient by the contaminated duodenoscopes.

Of significant concern to many labs and infection control departments are how to monitor reprocessed duodenoscopes for microorganisms that may survive the decontamination process and what actions should be taken if organisms are found.

The FDA and CDC recently released several communications and interim guidance documents concerning the current issues with duodenoscopes. The CDC has provided an interim guidance for epidemiological and surveillance studies to find the root cause. Rather than focus attention solely on the detection of CRE, the CDC recommends labs look for the presence of large numbers of bacteria and for “high concern” organisms. The high concern organisms are not normal skin or environmental flora and represent potential pathogens, such as



E. coli

, and



The method provided by the CDC includes a method for presence/absence testing as well as a quantitative culture method for obtaining colony counts from samples. Links to the CDC interim guidance documents are provided below.

Newly updated cleaning instructions from Olympus, the major manufacturer of duodenoscopes, is referenced below.

Olympus now recommends raising and lowering the elevator mechanism three times while the device is immersed in a disinfectant.

In addition, an area around the elevator (pictured above) must be cleaned with two different-sized brushes, one that hospitals already use and one that Olympus will ship by May 8.

Reprocessing of re-usable medical devices is an issue the FDA has been working on for several years. In 2011, the FDA issued a draft guidance on reprocessing medical devices. The FDA continues to work with medical device manufacturers, the CDC, and several other partners to improve medical device reprocessing. More information and guidance on medical device reprocessing can be found in the links below.

These recent outbreaks further highlight the need to combat antibiotic resistance. President Obama made antibiotic-resistant organisms a top priority for his administration by increasing federal funding to prevent antibiotic-resistance to $1.2 billion by 2016. The president also signed

Executive Order 13676

in September of 2014 to aid in the detection, prevention, and control of antibiotic resistant organisms.

For more information from the FDA and CDC, please check online at


or follow the additional links below.

Reusable Device Reprocessing Resources for Health Care Facilities

Reprocessing Medical Devices in Health Care Settings: Validation Methods and Labeling

Endoscopic Retrograde Cholangiopancreatography (ERCP) Duodenoscopes: FDA Safety Communication – Design May Impede Effective Cleaning

Interim Duodenoscope Surveillance Protocol

Interim Duodenoscope Sampling Method

Interim Duodenoscope Culture Method

New Cleaning and Reprocessing Instructions from Olympus

What was this man’s revolutionary idea

that has saved

millions of lives?

Optical oddities…

What is it?

Scroll to the bottom of this column for answer.

“Believe half of what you see

and none of what you hear.”

~ Marvin Gaye ~


Rubes Oct 2012

Find more

of Leigh Rubin’s cartoon humor.

Want to

book Leigh

as a speaker at your next event?

Pre-reduced Culture Media…



Hardy’s AnaeroGRO ensures superior recovery of anaerobic bacteria. The plates are packaged in a nitrogen gas flushed pouch that also includes an oxygen scavenger sachet.


more about AnaeroGRO.


a sample for evaluation.
AnaeroGRO Logo

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New Reference Guide for Microbiologists!

Useful and practical  information on 2,100 species of bacteria at your fingertips!

As of 2015, the field of Clinical Microbiology has evolved into a complex world of new and exotic organisms which increasingly challenge the patient, the health care worker and the diagnostic laboratory.  Over 1,700 species of bacteria and yeasts have been identified in humans, and an average of one new organism is added to the list every week!

Bacteria which were previously considered to be “innocent bystanders” that inhabit the intestinal tract or skin are increasingly being described as significant pathogens, often the result of immune suppression or complex advances in treatment.This same technology has also resulted in changes in the names of several well established and familiar species.

Gideon Guide to Medically Important Bacteria

contains 1,825 pages as a 7 MB PDF.  Since this is an e-book, users can rapidly access a specific organism by name or former name (currently 2,100 listed) using standard search tools.  Unfortunately, a new organism described this week will appear in standard Microbiology texts and Laboratory identification systems many months or even years following publication.  Thus the Gideon Guide incorporates scores of new organisms and name designations which might be missed by most clinical laboratories.

The book is an A-to-Z reference that summarizes the practical features of all bacteria associated with human medicine.  This exhaustive text includes distinctive laboratory characteristics, ecology and clinical significance, previous taxonomic designations (“synonyms”) and full phenotype (biochemical reactions). This invaluable reference guide has a temporarily reduced price of $39.99.

Use the code “hardy10” and receive an additional 10% discount.

See a sample page.

Place your order now

. Be sure to use the discount code.

Whoever snuck the ”


into FA



Well done!


Pun Zone Ahead

  • Police were summoned to the daycare center where a three-year-old was resisting a rest.
  • When you’ve seen one shopping center, you’ve seen a mall.
  • Did you hear the one about the fellow whose entire left side was cut off? He’s all right now.
  • A bicycle can’t stand alone. It’s just two tired.

“Be ashamed to die

until you have won

some victory

for humanity.”

Horace Mann

1796 ~ 1859

Politician and Educator

Tireless promoter of public education.

Online Ordering Made Easy!

Online ordering


a short video

to learn how easy it is

to order from Hardy on-line!


. Click. You’re Done!

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Our Optical Oddity for this month is a photo of a lenticular cloud. This is a stationary lens-shaped cloud that often times above a mountain or large structure on a windy day. Sometimes the gullible will mistake it for a UFO (unidentified flying object). Aircraft pilots will avoid lenticular clouds, but glider pilots gleefully seek them out to ride the updrafts that surround them.

Here are several lenticular clouds forming over Mt. Rainier in Washington state.