a culture of service…
© 2017, Hardy Diagnostics,
all rights reserved
Color change makes it easy!
Now there’s an easy way to detect dermatophytes from hair, skin,
and nail specimens.
Request Free Samples
Keep the stain where it belongs…
Hardy’s GramPRO 1 will rapidly stain your smears and get it right every time! Our patented technology judges the thickness of the smear and adjusts the decolorization step to perfection. Not only that, there are no messy clean-up or maintenance procedures. The GramPRO 1 will produce a perfect slide every time in about 4 minutes.
a brief video on GramPRO 1.
the brochure on all of Hardy’s automated stainers.
contact me to discuss automated slide stainers.
The next generation…
Advanced Gram Stain
See for yourself how Hardy’s Advanced Gram Stain Kit will produce brighter, more distinct colors. Try our kit and compare!
* * *
Hardy is proud to announce its 2017 catalog of all culture media offerings.
This 85 page booklet contains descriptions of the 2,700 products that Hardy manufactures for the microbiologist.
the digital version.
an account rep contact me about a price quote.
* * *
The simple way to test for
E. coli and coliforms in water.
What is Hardy all about?
a short video to find out…
It’s time for a brain massage…
Think about it…
* Why do they put locks on the doors of 24 hour stores?
* Ever wonder why they sterilize the needles for lethal injections?
* How do you KNOW it’s new and improved dog food?
* What do they use to ship Styrofoam?
* Why is it called rush hour when everything moves so slow?
* * *
Wisdom to Ponder…
1757 ~ 1804
As one of the founding fathers of the United States, he was the first Secretary of the Treasury, founder of the national bank, and a promoter of a strong federal government. Tragically, he died at 47 after a pistol duel with Aaron Burr, leaving behind eight children.
“It’s not tyranny we desire; it’s a just, limited, federal government.”
“There is a certain enthusiasm in liberty that makes human nature rise above itself, in acts of bravery and heroism.”
“I never expect to see a perfect work from an imperfect man.”
“Nobody expects to trust his body overmuch after the age of fifty.”
“Our great error is that we suppose mankind to be more honest than they are.”
“People sometimes attribute my success to my genius; all the genius I know anything about is hard work.”
“When a government betrays the people by amassing too much power and becoming tyrannical, the people have no choice but to exercise their original right of self-defense – to fight the government.”
“Give all the power to the many, they will oppress the few. Give all the power to the few, they will oppress the many.”
* * *
Online Ordering Made Easy!
a short video
to learn how easy it is
to order from Hardy on-line!
Pick. . . Click. . .
And your order is on its way!
* * *
Did you know?
- Is celebrating its 36th year of serving microbiologists.
- Manufactures from three ISO certified factories; one in California, Ohio, and Texas.
- Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
- Services over 10,000 labs and maintains a worldwide network of over 80 distributors.
Want to receive the MicroBytes
Newsletter at home?
Want to view past issues
“As Hardy Diagnostics enters its 37th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
“I don’t mind my wife having the last word. In fact, I’m delighted when she gets to it.”
* If attacked by a mob of clowns, go for the juggler.
* I’m still hot. It just comes in flashes now.
* The first five days after the weekend are the hardest.
* I child proofed my house, but the kids still get in.
* Ban pre-shredded cheese! Make America grate again.
* The past, present and future walk into a bar. . . It was tense.
# # #
“There are times when parenthood seems nothing but feeding the mouth that bites you.”
You have heard of
but do you know about
It has to do with
shredded cabbage and mayo…
G. vag and UTIs
Does Gardnerella vaginalis
play an important role
in Urinary Tract Infections?
A vaginal epithelial cell infected with multiple Gram negative and positive bacterial species including G. vaginalis, indicative of Bacterial Vaginosis (BV).
Urinary tract infections are a result of bacteria entering the urinary tract through the urethra. When these bacteria arrive at the bladder, they begin to multiply, leading to an infection. In most cases, the urinary system can usually fight off these invaders; but in cases where the defense of the urinary system fails, UTIs affect the bladder and urethra.
When the infection persists in the bladder, it is typically caused by Escherichia coli. This particular type of infection is referred to as “cystitis.” In cases where the infection is found in the urethra, a result of gastrointestinal (GI) bacteria spreading from the anus to the urethra, this particular type of infection is called “urethritis.”
While E.coli has historically been the primary culprit of UTIs, studies show that the vaginal bacterium, Gardnerella vaginalis, plays a significant role. G. vaginalis is a gram-variable, facultative anaerobic bacterium that is non-motile and non-sporeforming. It is commonly associated with bacterial vaginosis (BV) when it persists in the vagina and overwhelms the healthy Lactobacillus population, especially in young, sexually active females. It is now accepted that Gardnerella vaginalis is not the sole cause of the condition, which is characterized by a reduction in the numbers of Lactobacilli and an increase in other bacteria including: Gardnerella vaginalis, Bacteroides spp, Mobiluncus spp, anaerobic Streptococci, Mycoplasma hominis and Ureaplasma urealyticum. The term Bacterial Vaginosis better reflects the diverse vaginal microbiology of this dysbiotic condition. On a gram stain, a vaginal smear generally shows a shift from Gram positive to predominantly Gram negative bacteria.
Recent studies show that G. vaginalis aids E. coli in its infection of the bladder. Researchers at the Washington University School of Medicine in St. Louis infected bladders of female mice with E. coli to initiate UTIs. When these mice recovered from the infection after one month, their urine was screened for E. coli, but none were detected. This screening was performed because former studies had shown that some E. coli persist in the bladder even post-infection and are found in very low levels in urine. After the infection had cleared and no E. coli was detected in the urine, researchers introduced either Lactobacillus crispatus or G. vaginalis, both residents of normal vaginal microflora, to the bladders of the mice. These bacteria were eliminated from the bladder within 12 hours of introduction. Interestingly, however, E. coli appeared in the urine of more than half of the female mice that were introduced to G. vaginalis.
A bladder epithelial cell (blue) that has been exposed to G. vaginalis is in the state of dying and detaching from its neighboring cells (teal), revealing immature cells below (purple).
Credit: M. Joens, J. Fitzpatrick and N. Gilbert, Washington University, School of Medicine, St. Louis.
With further speculation, it appears that G. vaginalis plays a role in the recurrence or “awakening” of E. coli in the bladder and, thereby, aids in recurrent infections. One quarter of women treated for UTI will experience a recurrence within six months.
It was found that some of the mice exposed to G. vaginalis had infections that moved from the bladder, up through the urinary tract, and into the kidneys, causing a kidney infection. Researchers at the university suggest that G. vaginalis may damage cells on the surface of the bladder, releasing the dormant E. coli housed in those bladders cells. As a result, when the dormant E. coli cells are liberated, they can begin to multiply and contribute to recurrent UTIs.
There are some major implications of this study which impact the diagnosis and treatment of UTIs. Of concern is the fact that the antibiotics commonly used to treat UTIs are not effective in eliminating G. vaginalis.
Moving forward, it may be important for physicians and practitioners to consider a patient’s history of bacterial vaginosis and the presence of G. vaginalis in a UTI sample. Historically, G. vaginalis in a UTI sample may have been disregarded as a contaminant of the normal vaginal microflora. However, this study suggests that G. vaginalis may play a significant role in recurrent UTIs.
by Nasim Delavari, Technical service, Hardy Diagnostics
* * * * *
Winner of the ASM’s
Agar Art Contest
The winner, Md Zohorul Islam, DVM, from Denmark created this elaborate depiction of fertilization. The winning sperm is seen to penetrate the egg and is heading for the nucleus. For this entry of Agar Art, he used Staphylococcus aureus (red), Staphylococcus xylosus (green), Staphylococcus hyicus (white), and Corynebacterium glutamicum (yellow).
the winners of the ASM’s Agar Art contest from last year in Microbe World.
The second place winner was Mariarosaria Marinaro, PhD, from Italy, who created a beer mug from MRSA with the help of catalase to produce the foam.
* * * * *
Do Bacteria Behave
Differently in Outer Space?
Much work is needed to determine the characteristics of bacteria growing in a weightless atmosphere. There is growing concern about enhanced bacterial growth and resistance in outer space.
Consider the following…
1. NASA discovered in the 1960s that E. coli and Salmonella grow twice as fast when orbiting in the space module.
2. Soviet scientists in the 1970s found that bacteria developed increased resistance to five antibiotics while in orbit.
3. Cosmonauts on the Soviet space station Mir, which was operational from 1986 to 2001, endured conjunctivitis, food poisoning, and acute respiratory illnesses.
4. In 2006, Cheyl Nickerson from Arizona State University found that bacteria became more lethal to lab animals in space than on Earth.
5. During NASA’s space shuttle program, from 1981 to 2011, 29 astronauts officially reported severe head colds, fevers, stomach ailments, or other nagging infections while in orbit; none of them were life-threatening. Among all shuttle crew members, 75% took medications for minor ailments during their space flights.
6. A study at the University of Colorado last year compared E. coli bacteria on the space station with those on Earth. Their results show cells grew 13 times faster in orbit and that dozens of E. coli genes were more active in space than on Earth.
The cramped interior within the International Space Station creates a concern, especially when astronauts experience an altered microbiome after extended periods of time in space.
* * * * *
Increase your recovery rate for anaerobes!
Hardy’s AnaeroGRO is packaged with an oxygen scavenger and flushed with nitrogen gas to ensure anaerobic conditions, leading to better recovery of anaerobic bacteria.
anaerobic microbiology supplies.
* * * * *
C diff Banana Broth
With nearly 500,000
C diff infections annually…
Will your infection control
protocols hold up?
Hardy announces a new medium for the detection of
Clostridium difficile bacteria and spores. This new medium is capable of saving hospitals many thousands of dollars. It is useful in monitoring the efficacy of cleaning procedures used in patient rooms.
This is the first and only culture medium designed for the detection of C. difficile and its spores on surfaces in a hospital setting. The specificity was found to be 100% in a recent study (see reference below). No special equipment or anaerobic supplies are needed! Obtain results in as little as 24 hours!
View a short video explaining how C. diff Banana Broth can increase the level of patient safety and save your hospital many thousands of dollars! Study showing 100% specificity (no false positives).
* * * * *
First Place Winner of the Nikon
“Small World in Motion” Contest…
this amazing video of a Trachelius ciliate devouring a Campenella ciliate.
ciliates are a group of protozoans characterized by the presence of hair-like organelles called cilia. Ciliates are an important group of protists, common almost everywhere there is water – in lakes, ponds, oceans, rivers, and soils. About 3,500 species have been described, and the potential number of total species is estimated at 30,000.
* * * * *
Two heads are better than one!
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Testimonial from a Pharmaceutical Lab worker…
“The two heads of TRIO BAS DUO air sampler was one of the best investments during the last two years. The laboratory staff responsible of the bacteriological sampling is able to double the number of environmental microbial cycles per day. This means more efficiency and lower cost, together with the possibility to increase in the future the number of sampling in other areas of the premises.”
Do Not Disturb!
Fungal slide cultures made easy!
Hardy’s MycoVue takes out all the tedious labor of preparing a professional slide culture.
Just inoculate the agar cube, place the cover slip on top, incubate, and place the entire unit on the microscope stage for easy examination.
The fungal culture can be observed without disturbing the delicate structures.
If you have need of animal blood, think of Hardy. Our sheep are bled in a humane way with a veterinary supervised program. Many sizes and container configurations are available.
a sales rep call me.