Partnering with microbiologists to diagnose and prevent diseaseTM

 

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a culture of service…

December, 2017

 

© 2018, Hardy Diagnostics,

all rights reserved 

 

 

 

Hardy…your one stop shop for…

 

 
Veterinary 
Microbiology
See our online catalog.
Request our paper catalog.
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For the detection

 

 of Group B Strep…
 
Carrot Broth One Step
  • Improved…No tile addition needed!
  • Detects hemolytic Group B Strep from the initial broth culture
  • Provides results in as little as sixteen hours
  • Found to be 100% sensitive and up to100% specific in a recent study
 
 
Request samples.
 
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Gram Staining made easy…
 

No Mess!
No Stress!
Hardy’s GramPRO is the world’s most consistent, repeatable, and reliable way to perform a Gram stain. Find out why…

 

 

Watch a brief video about how easy it is to set up the GramPRO in your lab.
Learn more about the GramPRO 1.

 

 

Please contact me to discuss automated slide stainers.

 

 

 

 

New Kirby Bauer Discs

 

Avycaz and Zerbaxa
Now Available

 

Mueller Hinton
Avycaz (Ceftazidime/Avibactam) and Zerbaxa (Ceftolozane/Tazabactam), two of the newest antibiotics to be cleared by the FDA, are now available as Kirby-Bauer disks from Hardy Diagnostics.
View our entire AST Mini Catalog
Also available: Vabomere (Meropenem/Vaborbactam)

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Phraseology…
“Stool Pigeon”
The original Stool Pigeons were real pigeons that were perched on a stool. Pigeon hunters long ago would tie down a pigeon to a stool. It would then flap its wings and attract other pigeons that would get caught in the hunter’s net. Thus, Stool Pigeon became known as a term for an informer or decoy.
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What is Hardy all about?
View a short video to find out…

 

 

 

 

 

 

 

 

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Brainteasers

Brain


Lazy minds need not apply.
Active minds enter here

StrepPRO

 

Rapid ID of Strep Grouping 

by Latex Agglutination

 

StrepPRO  

Learn more and order

See all our rapid tests

 

Maude on her cell phone: Harold, please help me. My car won’t start.

Harold: It could be due to the the cold weather we are having.

Maude: I don’t think so. My dashboard is giving me some kind of odd warning.

Harold: What is it telling you?

Maude: I’m not sure; it’s just an image of a man sitting on a toilet! What could that mean?

Scroll to bottom to see the image that Maude saw on her dashboard this last week….

Think about it…
    
The Thinker

 

 

* The letter W, in English, is called double U. Shouldn’t it be called double V?
* Maybe it’s oxygen that is slowly killing us. It just takes 75-100 years to fully work.
* Every time you clean something, you just make something else dirty.
*The word “swims” upside-down is still “swims.”
*Intentionally losing a game of ‘rock, paper, scissors’ is just as hard as trying to win.

 

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Wisdom to Ponder…
 
     
Helen Keller
1880 ~ 1968
Having gone blind and deaf at 19 months of age, she later became a famous American  author, lecturer, and political activist. 

 

“The only thing worse than being blind is having sight but no vision.”
“Alone we can do so little; together we can do so much.”
“Life is either a great adventure or nothing.”
“The best and most beautiful things in the world cannot be seen or even touched – they must be felt with the heart.”
“Walking with a friend in the dark is better than walking alone in the light.”
“What I am looking for is not out there…it is within me.”
“Life is an exciting business, and most exciting when it is lived for others.”
“All the world is full of suffering. It is also full of overcoming.”
“It’s wonderful to climb the liquid mountains of the sky. Behind me and before me is God, and I have no fears.”
“People do not like to think. If one thinks, one must reach conclusions. Conclusions are not always pleasant.”

“No one has a right to consume happiness without producing it.”

 

 

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Online Ordering Made Easy! 

 

 

 

Online ordering

Watch a short video
to learn how easy it is
to order from Hardy on-line!

 

Pick. . . Click. . .
And your order is on its way!

 

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Christmas is just like work.

You do all the work and 
a fat guy in a suit gets all the credit! 

 

 

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Did you know? 
 
Hardy Diagnostics…
  • Is celebrating its 38th year of serving microbiologists.
  • Manufactures from two ISO certified factories; one in California and one in Ohio.
  • Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
  • Services over 10,000 labs and maintains a worldwide network of over 80 distributors.
  • Is a 100% Employee-Owned company. “If we act like we own the place…it’s because we do!”

 
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QUICK LINKS…
 

 

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Want to receive the MicroBytes
Newsletter at home?

 

 
Want to view past issues
of MicroBytes?
 

 

 

“As Hardy Diagnostics enters its 38th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
Jay Hardy, CLS, SM(NRCM)
President
HARDY DIAGNOSTICS

 

Jay's photo 2

 

 
For your ordering convenience!
 
 
Did you know that of the 2,700 products that Hardy makes, 700 of them are now available on Amazon.

 
Micro Musings…
 

“Yes, I know that there are trillions of microbes
living on my body…but I still get lonely sometimes.”

Do You Perform Growth Promotion Studies?
EZ Accu Shot
  
Hardy offers a complete line of control organisms for quality assurance. The EZ Accu Shot offers quantitative microorganism testing for growth promotion studies.

 

Learn more about Accu Shot…
Learn more about Growth Promotion products…
See our entire line of microorganisms…
 
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Cannabis Testing
  
Hardy offers many products for the microbiological testing of cannabis. See the related products here
  
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More exciting discoveries coming soon!
 

  

iChip and the 99%

 

 

A new device called the iChip, short for isolation chip, may unlock the potential to culture the majority of microbes that still remain undiscovered. 

 

So far, standard microbiology culture methods have only been able to grow about 1% of microbial species in vitro on synthetic media (1). Beginning in 2002, the iChip was designed and tested by a team of researchers from multiple universities and industry partners and eventually completed its phase I (proof of concept) design in 2010. 
 
The iChip is capable of isolating and growing pure microbial cultures at a high throughput. With the ability to recover more organisms that were previously unable to be cultured by current methods, the iChip has the potential to improve the present understanding of environmental microorganisms and will allow for new discoveries that are applicable to various areas of biotechnology and medicine (2).

 

What makes the iChip unique is that it allows for bacteria to remain in their “natural environment” by bringing the environment to the lab. The iChip is capable of culturing organisms from soil, sea water, saliva, salt marsh, and wastewater bioreactors (1,2,3).

 

In order to accomplish this, the iChip utilizes a “chip,” which consists of a hard piece of plastic with 192 tiny wells.  To use this device, the user simply dips the unit in a bacterial sample mixed with agar  to trap a single cell in each well.  After collection of the bacterial sample, a diffusion membrane is placed on each side of the chip in order to lock the microbes in place. The membranes are subsequently secured with plastic plates, and the iChip is placed into a large sample of its original environment where nutrients may permeate through the membranes. This has been shown to allow for an observed 30,000% increase in bacterial growth compared to standard agar plates (1).
 

 
Thanks to the iChip, the team has analyzed over 10,000 microbial species in a simple one-step process. As a result, the team discovered 25 new antibiotics in 2005. The antibiotic with the most promise was isolated from a recently discovered organism called Eleftheria terrae. The new antibacterial molecule, named teixobactin, was successful in wiping out highly drug-resistant microbes responsible for anthrax and tuberculosis (4,5). Teixobactin has been observed to protect mice from bacteria that are non-susceptible to several different drugs, including methicillin-resistant Staphylococcus aureus (MRSA) (4).

 

This new antibiotic is already being chemically synthesized in two simplified versions using methods that may be reproduced on a commercial scale at the University of Lincoln, UK, for an economical and commercially available product for use in human clinical trials (4). Researchers believe that antimicrobial resistance is less likely to arise against these new synthetic antibiotics because the enzyme that the drug targets would require significant structure changes to become resistant (4,5).

 

With the ability to culture microbes quickly, testing the iChip in other environments may help identify additional molecules or proteins from unidentified organisms that may have medical or industrial value.
 
This new technology provides the possibility to recover microbes from difficult to reach and complex environments (e.g., outer space, deep ocean trenches, etc.). Advances in the iChip leave developers with high aspirations; the potential for expansion of new beneficial molecules is imminent. Standard culture methods that utilize agar or broth media often produces little to no growth, due to the lack of nutrients or growth promotion factors yet to be discovered.

 

The majority of molecules discovered thus far come from the 1% of bacteria known to exist. Now, the potential to tap into the remaining 99% is exciting for all avenues of scientific research.
References:
1, 2, 3, 4, 5, 6

 

by Pancho Ibarra
Technical Support Microbiologist
HARDY DIAGNOSTICS
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Trio Bas
Air Samplers
 
Two heads are better than one!
 
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Testimonial from a Pharmaceutical Lab worker…
 
The two heads of TRIO BAS DUO air sampler was one of the best investments during the last two years. The laboratory staff responsible of the bacteriological sampling is able to double the number of environmental microbial cycles per day. This means more efficiency and lower cost, together with the  possibility to increase in the future the number of sampling in other areas of the premises.”
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Increase your recovery rate for anaerobes!
AnaeroGRO
   anaeroGRO

 

Hardy’s AnaeroGRO is packaged with an oxygen scavenger and flushed with nitrogen gas to ensure anaerobic conditions, leading to better recovery of anaerobic bacteria.

 

See the short video.
Request a free sample.
Learn more about the benefits of AnaeroGRO.
View a catalog of all our
anaerobic microbiology supplies.
 
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Proceed with caution!

 

Fluoroquinolone Resistance
and
Screening Methods
Fluoroquinolones have been the staple of antimicrobial therapy since the launch of the flagship representative “Ciprofloxacin” by Bayer in the early 1980s.
Fluoroquinolones have a fluorine atom attached to the core quinolone molecular ring and this modification results in a class of broad-spectrum bactericidal agents that show increased stability and potency against both Gram negative and Gram positive organisms.
In the years since its introduction, many other fluoroquinolones have been cleared for use, such as gemifloxacin, levofloxacin, moxifloxacin, and ofloxacin, among others. As predicted, resistance to fluoroquinolones has increased in the last decade, as the majority of fluoroquinolones were introduced in the late 1990s. For instance, in large surveillance programs like SENTRY, data from 2013 to 2016 show the resistance rates at 15.6% and 18.6% for general Enterobacteriaceae and
Pseudomonas populations, respectively. However, the resistance rates are significantly higher in multi-drug resistant subsets (ref. 1). In the May 2016 edition of
Antimicrobial Agents and Chemotherapy publication by Bidell et al., the rate of resistance in Escherichia coli strains from hospital-associated urinary tract infections range from 25% to 43% (ref. 2). These recent data certainly support the importance of screening for non-susceptible isolates in patients prior to administering the drug for treatment.
Generally, resistance to fluoroquinolones occurs through step-wise mutations, which are a series of point mutations that occur over time in the gyrase or topoisomerase gene that alter the bacterial enzyme structure to make the fluoroquinolone less effective or ineffective at binding and disabling the enzyme. When a new fluoroquinolone is administered, it may be effective at killing the bacteria at first. However, point mutations that lead to a beneficial change in enzyme structure are selected for which facilitates resistance against the new drug. Each new fluoroquinolone presents a new selective pressure that a previous fluoroquinolone may not have presented. However, mutations can accumulate one at a time or in a step-wise manner, leading to increasing fluoroquinolone resistance with each successful mutation (ref. 3).
Fluoroquinolones are widely administered for urinary tract infections, pre- and post-prostate biopsy, and hospital or community acquired infections that show multi-drug resistance. However, the use of fluoroquinolones has been cautioned against as there have been cases linked to devastating side effects that involve the central nervous system and tendon rupture (especially the Achilles tendon).
When other treatment options are available, they should be followed before treating with fluoroquinolones, as this class of antibiotics has been observed to have a risk of debilitating side effects, even during a short treatment period. Therefore, the FDA has warned against using fluoroquinolones for sinusitis, bronchitis, and uncomplicated urinary tract infections. (ref. 4).
The practice of screening for fluoroquinolone resistant E. coli prior to transrectal prostate biopsy procedures to ensure the success of fluoroquinolone prophylaxis and to reduce the risk of infection was described by Dr. Liss in the Journal of Clinical Microbiology in 2011.
In essence, the procedure calls for inoculation of stool or rectal specimen into a selective pre-enrichment broth or plated media that contains a fluoroquinolone at the non-susceptible breakpoint, resulting in the triage of cases that may potentially involve fluoroquinolone non-susceptible isolates (ref. 5). The resistance phenotype is subsequently confirmed via conventional susceptibility methods (ref. 6).
While the resistance tide is constantly against us from all angles involving all types of resistance (ESBL, KPC, MRSA, VRE, Polymyxin resistance), increased awareness of risks associated with infection complications from ciprofloxacin resistant E. coli can certainly be decreased with a simple  screening for resistance.

by Anna Klavins

 
R&D Microbiologist
HARDY DIAGNOSTICS
 
 

 

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   Optical oddities… 
 
Which do you see?
A lion or monkey?

 

 

 

 

  “Believe half of what you see

and none of what you hear.”

 ~ Benjamin Franklin ~

 

RUBES

Find more

Want to book Leigh as a speaker at your next event?

  “You had a great life,

but too bad you missed it by

looking down at your phone all the time.”

 
 
“Don’t walk behind me; 
I may not lead. 
Don’t walk in front of me; 
I may not follow. 
 
Just walk beside me and be my friend.” 
 
 

 

Warning:
PUN ZONE
AHEAD
* I changed my iPod’s name to ‘Titanic.’ It’s syncing now.
 
Venison for dinner…again? Oh deer!
* England has no kidney bank, but it does have a Liverpool.
* I tried to catch some fog, but I mist.
* The lab told me I had type-A blood, but it was a Typo.

 

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Think about it…
Hospitals are full of people
Having the worst day of their life,
Having the best day of their life,
Having the last day of their life, or
Having the first day of their life!