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A breakthrough in new antibiotics!

 

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a culture of service…

May, 2017

 

© 2017, Hardy Diagnostics,

all rights reserved 

 

 
Attention Mycologists…
     
BlueMount
Make beautiful permanent slides for fungi!
 

BlueMount combines Lactophenol Cotton Blue and Polyvinyl Alcohol to make fungal slides that will last a lifetime!
View the product information.
Browse all of our Mycology supplies.
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Load 80 slides at a time!
 

No Mess!
No Stress!
Hardy’s GramPRO 80 will rapidly stain your smears and get it right every time! With the GramPRO 80 you can load up to 80 slides at a time, while it processes about 60 slides per hour. Also, there is a STAT function so you can easily insert a slide that needs to be done right away.

 

Our patented technology judges the thickness of the smear and adjusts the decolorization step to perfection.

 

Not only that, there are no messy clean-up or tedious maintenance procedures.

 

For smaller labs, the GramPRO 1 will accept and process one slide to perfection within four minutes.

 

 

 

Find out more about all Hardy stainers.

 

Watch a brief video on GramPRO 80.

 

Watch a brief video on GramPRO 1.

 

View the brochure on all of Hardy’s automated stainers.

 

 

Please contact me to discuss automated slide stainers.

 

 

 

 

Showing 2,700 products!

 

Hardy’s Culture
Media Catalog

 

Hardy is proud to announce its 2017 catalog of all culture media offerings.
 
This 85 page booklet contains descriptions of the 2,700 products that Hardy manufactures for the microbiologist.
View the digital version.
Send me the paper version.
Please have an account rep contact me about a price quote.

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Screening for STEC?
 
SHIBAM!
 
Need a simple and inexpensive way to detect shigatoxin from food and environmental samples? 

 

 

 

Plate on the left shows hemolysis from an STEC strain of E. coli. Plate on the right shows a non-pathogenic E. coli.

 

 

 

 

Hardy’s SHIBAM plates will screen for all six of the main STEC serotypes!

 

 

 

 

View the FDA’s BAM procedure.
View the product information.
Request a sample.

A military poster published during WWII, when the standard treatment for syphilis was either a mercury or arsenic compound. Penicillin did not became widely used for syphilis until after the war.

 

 

 

 

 

 

 

 

What is Hardy all about?
View a short video to find out…

 

 

 

 

 

 

 

 

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Brainteasers

Brain

  

In need of a cranial workout?
Feel the burn here

Think about it…
    
The Thinker

 

* Isn’t the best way to save face is to keep the lower part shut?
* Sooner or later, doesn’t EVERYONE stop smoking?
* A bus station is where a bus stops. A train station is where a train stops. On my desk, I have a work station…
* Can atheists get insurance for acts of God?
* If FedEx and UPS were to merge, would they call it Fed UP?
* Does fuzzy logic tickle?

 

 

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Wisdom to Ponder…
  


Oscar Wilde
1854 ~ 1900

 

Irish playwright, novelist
poet, and essayist

 

“I can resist everything except temptation.”

 

“Experience is simply the name we give our mistakes.”

 

“True friends stab you in the front.”

 

“Women are made to be loved, not understood.”

 

“Some cause happiness wherever they go; others whenever they go.”

“I am so clever that sometimes I don’t understand a single word of what I am saying.”

 

“Everything popular is wrong.”
“Ordinary riches can be stolen; real riches cannot. In your soul are infinitely precious things that cannot be taken from you.”
“Life is never fair, and perhaps it is a good thing for most of us that it is not.”

“The old believe everything; the middle-aged suspect everything; the young know everything.”

 

“Whenever people agree with me, I always feel I must be wrong.”

 

 
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Online Ordering Made Easy! 

 

 

 

Online ordering

Watch a short video
to learn how easy it is
to order from Hardy on-line!

 

Pick. . . Click. . .
And your order is on its way!

 

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Did you know? 

 

 
Hardy Diagnostics…
  • Is celebrating its 37th year of serving microbiologists.
  • Manufactures from three ISO certified factories; one in California, Ohio, and Texas.
  • Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
  • Services over 10,000 labs and maintains a worldwide network of over 80 distributors.
  • Is a 100% Employee-Owned company. “If we act like we own the place…it’s because we do!”

 
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“As Hardy Diagnostics enters its 37th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
Jay Hardy, CLS, SM(NRCM)
President
HARDY DIAGNOSTICS
Jay's photo 2

 

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“Education is what remains after one has forgotten what one has learned in school.” 
Albert Einstein

 

 

Warning:
PUN ZONE
AHEAD
 
* I really wanted a camouflage shirt, but I couldn’t find one.
* Novice pirates make terrible singers because they can’t hit the high seas.
* Einstein finally developed a theory about space, and it was about time too.
* How do they figure out the price of hammers? Per pound.
* Why did the pig stop sunbathing? He was bacon in the heat.

 

#  #  #

 

“Youth is wasted on the young”
George Bernard Shaw
 

 

Q: Why does Waldo wear 
a striped shirt?
A: Because he doesn’t 
want to be spotted.
Micro Musings…
 
   “Sometimes I wonder if there’s more to life than unlocking the mysteries of the universe”
 

Botulism
Found in Nachos
In late April of this year, there was an outbreak of botulism due to contaminated nacho cheese at a gas station outside of Sacramento, California. The issue is no longer a threat, as the lot of cheese has been recalled and was discarded on May 5, 2017. However, the outbreak took its toll on nine people which were hospitalized, and one person died. Incidents such as these remind us of the importance of proper food preparation and preservation.
Seemingly innocent gas station food proved to be deadly in Sacramento.
Botulism is caused by the bacteria Clostridium botulinum. This Gram-positive organism has caused documented cases of illness dating back to the 18th century. When we think of preserved foods, we usually think they are safe. After all, isn’t that what preserved means? However, low oxygen conditions can promote germination of this spore-forming anaerobic organism.  Improperly processed canned and jarred foods are some of the most common culprits. However, as we see with the nacho cheese case, this can be found in any container that lacks oxygen.
Another cause for infection from botulism is from wounds, often relating to intravenous drug use.
Of all the documented cases, the most common occurrence of botulism is actually in infants. Infants who ingest the spores become colonized in the gut. Honey has been linked to a majority of these cases, and therefore, it is advised that honey not be fed to children less than 1 year of age.
Although botulism infections usually last one to ten days, some cases can become more severe. The man who died in the nacho cheese incident was in the hospital for several weeks on a ventilator before his eventual passing. While the disease is fatal in about three to five percent of cases, it can usually be treated with an equine-derived antitoxin. However, complications from using this antitoxin can arise, such as serum sickness and anaphylaxis. Therefore, it is not recommended for use with infants. Since infants are the most affected age group for disease, in 2003, a human-derived antitoxin was developed, giving a new treatment option for infants who have the misfortune of contracting this disease.
Contrary to popular belief, Clostridium botulinum itself is not actually what causes the illness; rather, it is the Botulinum neurotoxin it produces, which is the most lethal toxin known. The toxin, which is released upon sporulation, causes blurred vision, drooping eyelids, slurred speech, and even paralysis. Botulism toxin exists in seven different serological forms, which are labeled with the letters A-H. Types A, B, and F can be proteolytic, making detection easier since they give off a rotten odor. All serotypes affect humans, except C and D, which only affect animals. In the United States, virtually all reported cases have been due to types A and B.
Clostridium botulinum showing spore formation. Simple boiling cannot kill the spores. Increased heat under pressure is needed. 
Prevention of bacterial growth and its associated spores is not difficult. Though the nacho cheese travesty made headlines, cases usually arise from home-preserved foods. Hopefully, anyone that is practicing this hobby knows of the dangers associated with canning and jarring foods and usually understands the proper precautions that need to be taken. For people new to food preservation, the USDA and CDC lists some important instructions in home canning, including taking special care for foods that have low acid (pH > 4.6). Never eat from a can that is bulging or leaking. Never eat food that is foamy or smells bad. When in doubt, throw it out!
Being a fairly rare disease, the case in Sacramento was shocking to say the least. In 2015, there were 39 botulism cases linked to food. The largest outbreak was due to potato salad at a potluck where 27 people became ill. As we are coming up on summer, special precautions are needed to defeat this potentially devastating food-borne disease
By Trevor Thorsen
Technical Support Specialist
Hardy Diagnostics
Erbguth, F. J. (2004). Historical notes on botulism, Clostridium botulinum, botulinum toxin, and the idea of the therapeutic use of the toxin. Movement Disorders, 19(S8), S2-S6.
Black, R. E., & Gunn, R. A. (1980). Hypersensitivity reactions associated with botulinal antitoxin. The American journal of medicine, 69(4), 567-570.
Arnon, S. S., Schechter, R., Maslanka, S. E., Jewell, N. P., & Hatheway, C. L. (2006). Human botulism immune globulin for the treatment of infant botulism. New England Journal of Medicine, 354(5), 462-471.
Sobel, J., Tucker, N., Sulka, A., McLaughlin, J., & Maslanka, S. (2004). Foodborne botulism in the United States, 1990-2000. Emerging infectious diseases, 10(9), 1606.
McCarty, C. L., Angelo, K., Beer, K. D., Cibulskas-White, K., Quinn, K., de Fijter, S., … & Shafer, G. (2015). Notes from the field: large outbreak of botulism associated with a church potluck meal-Ohio, 2015. MMWR Morb Mortal Wkly Rep, 64, 802-3.

 
C diff Banana Broth
With nearly 500,000
C diff infections annually…
Will your infection control
protocols hold up?
Hardy announces a new broth medium for the detection of Clostridium difficile bacteria and spores. This new medium is capable of saving hospitals many thousands of dollars. It is useful in monitoring the efficacy of cleaning procedures used in patient rooms. Positives turn yellow!

 

This is the first and only culture medium designed for the detection of C. difficile and its spores on surfaces in a hospital setting.  The specificity was found to be 100% in a recent study (see reference below). No special equipment or anaerobic supplies are needed! Obtain results in as little as 24 hours!

View a short video explaining how C. diff Banana Broth can increase the level of patient safety and save your hospital many thousands of dollars!
 
 
 
Request a sample for your evaluation.
 
View the Study  showing 100% specificity (no false positives).
 
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Advances in 
Gram positive antimicrobials
New Superdrug
Beats Superbugs
A new and very potent weapon against VRE and VRSA has been developed that is at least 1,000 more active than vancomycin and leaves no chance for the development of resistance!
Antibiotic resistance has been extensively explored and has been a conundrum for the medical profession for several decades as bacteria find ways to break down and inactivate the most sophisticated compounds, while others go so far as to alter their metabolic pathways in order to circumvent antimicrobial targets.

 

While vancomycin has been a staple therapy since 1958 in the fight against Gram-positives such as Staphylococcus, resistance in enterococci is quite common. Enterococcus faecalis produces low-affinity penicillin binding protein (PBP), which makes it more difficult for penicillin to bind to this bacterium.  Other species of enterococci produce inactivating enzymes against aminoglycoside antibiotics, like streptomycin or gentamicin.  Vancomycin resistant Enterococci (VRE) express multiple phenotypes, specifically gene clusters of VanA, VanB, VanC, VanD, VanE, and VanG, that alter an amino acid sequence and prevent binding of vancomycin.

 

Traditionally, vancomycin inhibits synthesis of the peptidoglycan cell wall by binding to amino acid residues of specific peptides within Gram-positive bacteria; however, some species of enterococci are able to bypass this mechanism by altering this amino acid sequence using lactate (VanA, VanB, and VanD gene clusters) or serine (VanC, VanE, and VanG gene clusters) instead of the typical alanine.  The frightening aspect of this mechanism is the ability of these strains to transfer these resistance genes to other bacteria (even those from a different genus and species) via horizontal gene transfer.

 

This means bacterial genera normally inhibited by vancomycin could obtain resistance to it, potentially becoming multi-drug resistant if they already carry other resistance genes.

 

In a recent breakthrough study at The Scripps Research Institute, scientists have altered vancomycin in such a way to increase its antimicrobial potency.  In April, 2017, Dale Boger, PhD and his team were able to change the structure of this drug, nicknamed “Vancomycin 3.0,”  by adding two compounds to its periphery. This represents the first time that three different mechanisms of action were combined into one drug. This resulting “superdrug”  has a potency of 1,000 times that of its predecessor!
Dale Boger (right) and Akinori Okano (left) leading the  fight against resistant Gram positive bacteria at the Scripps Research Institute in San Diego, California.
Their modified vancomycin is the first antibiotic equipped with three different mechanisms of action, making it nearly impossible for bacteria to acquire resistance against the modified form.  A bacterium resistant to traditional vancomycin would have to overcome two additional mechanisms of action.  “This increases the durability of this antibiotic. Organisms just can’t simultaneously work to find a way around three independent mechanisms of action.  Even if they found a solution to one of those, the organisms would still be killed by the other two,” Dr. Boger explained.  In the lab, the modified vancomycin was able to inhibit both resistant and non-resistant strains of enterococci.

 

Vancomycin 3.0 has a complicated structure that combines three mechanisms of action (highlighted in green) against Gram positives.

 

 

The drug is not quite ready for clinical trials, since Boger’s team is attempting to find ways to reduce the expensive 30 steps required for its fabrication. To reach the market, it may take up to five years.
Antibiotic modification may be the beginning of a new fight against bacterial resistance.  If Dr. Boger’s team is able to perfect this modification process, who’s to say that it can’t be done with other antibiotics as well?

 

We may now have the tools to fight the dreaded “superbug,” and it shows thinking outside of the box may be a new and creative way to exploit available drugs that may be potentially improved.

 

by Sarah Hylton
Technical Support Specialist
Hardy Diagnostics
References:
Miller, William R., Jose M. Munita, and Cesar A. Arias. “Mechanisms of antibiotic resistance in enterococci.” Expert review of anti-infective therapy 12.10 (2014): 1221-1236.
Cetinkaya, Yesim, Pamela Falk, and C. Glen Mayhall. “Vancomycin-resistant enterococci.” Clinical microbiology reviews 13.4 (2000): 686-707.
Press Release: Scripps Research Institute. New antibiotic packs a punch against bacterial resistance. May 29, 2017. Retrieved June 07, 2017, from https://www.eurekalert.org/pub_releases/2017-05/sri-nap052517.php

 

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Increase your recovery rate for anaerobes!
AnaeroGRO
   anaeroGRO

 

Hardy’s AnaeroGRO is packaged with an oxygen scavenger and flushed with nitrogen gas to ensure anaerobic conditions, leading to better recovery of anaerobic bacteria.
See the short video.
Request a free sample.
View a catalog of all our
anaerobic microbiology supplies.
 
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“The kind of ‘assisted emigrant’ we can not afford to admit.” So reads the caption to this 1883 magazine drawing, which shows members of the New York Board of Health wielding a bottle of carbolic acid, a disinfectant, in their attempts to keep cholera at bay.
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Trio Bas
Air Samplers
 
Two heads are better than one!
 
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Testimonial from a Pharmaceutical Lab worker…
 
The two heads of TRIO BAS DUO air sampler was one of the best investments during the last two years. The laboratory staff responsible of the bacteriological sampling is able to double the number of environmental microbial cycles per day. This means more efficiency and lower cost, together with the  possibility to increase in the future the number of sampling in other areas of the premises.”
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   Optical oddities… 
 

 

 
How many legs does an elephant have?

 

 

 

 

  “Believe half of what you see

and none of what you hear.”

 ~ Benjamin Franklin ~

 

RUBES

 

Find more

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ComfortPRO
Labcoats

 Lab Coats
  • Three pockets: two hip and one breast
  • Laminated 3-ply for better protection
  • Knit collar and knit cuffs for comfort
  • Snap closures
  • Available in white or blue
  • Five sizes available: Small to XXL
  • Inexpensive and disposable
View the brochure.
 
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