a culture of service…
© 2016, Hardy Diagnostics,
all rights reserved
Pre-filled and ready to go…
Hardy’s Dilu-loks are pre-filled dilution blanks that save you the headache of making your own. The flip-lid makes it easy to open with one hand while you pipette with the other.
Available formulas include:
- Butterfield’s Buffer
- Phosphate Buffer with MgCl
- Buffered Peptone Water
- Sterile water
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The GramPRO 1
Hardy’s GramPRO 1 will rapidly stain your smears and get it right every time! Our patented technology judges the thickness of the smear and adjusts the decolorization step to perfection. Not only that, there are no messy clean-up or maintenance procedures. The GramPRO 1 will produce a perfect slide every time in about 4 minutes.
a brief video on GramPRO 1
the brochure on all of Hardy’s automated stainers.
contact me to discuss automated slide stainers.
New Culture Media Catalog
Hardy is proud to annouce its new catalog of all culture media offerings.
This 85 page booklet contains descriptions of the 2,700 products that Hardy manufactures for the microbiologist.
an account rep contact me about a price quote.
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Hardy offers a complete line of contact plates for your surface monitoring program. Whether you need irradiated or non-irradiated, single or triple bagged, Hardy has it in a variety of formulas.
a short video on EM.
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Last month, we published an article about Heat vs Methanol fixation for preparing microscope slides.
Here is a sampling of the comments we received…
” Methanol fixation is superior, especially from blood culture bottles. If you like I can send you two convincing examples of bottles with heat versus methanol.
They are images from a gram stain workshop I have presented at ASM and SWACM.”
teven D. Dallas, PhD, D(ABMM), MT(ASCP)SM
Associate Professor, Departments of Clinical Laboratory Sciences and Pathology
UT Health Science Center San Antonio, Texas
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“Jim Mangels convinced me back in the early 80s. He was working at Good Samaritan at the time.
I think most hospitals in the Bay Area (I hope) use methanol.”
O’Connor Hospital, California
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“I’ve worked in a hospital since 1971 and in Microbiology since 1972. During the early 1980’s I attended a wet workshop on anaerobes. Our instructor recommended methanol fixing for morphology. Once back in my lab, I experimented by making two slides on various specimen types with one receiving heat fixing and the other methanol fixing.
There was definitely an adjustment when reviewing our slides. Once we instituted methanol fixing as our method of choice, we observed a variety of positive findings. There was less debris on our slides; the morphology was a little crisper; great capsules; we found Giardia on our stool gram stains when we were looking for WBC.
I taught Microbiology seminar classes for a Community College for 27 years starting in 1980. Some of these students also rotated through our hospital for their lab training. Initially, some of the students were conflicted because I was teaching something different from their Microbiology class at the college. Their instructor inquired about our method. I continued to use methanol fixing in my lab and taught both methods to the students with explanations as to the why. Several years ago, the college found the methanol mentioned in a text and moved forward to teach the students both methods.
I appreciate the studies confirming what I already knew.”
Carol Frantz, Microbiology, Milford Hospital, CT
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Here is the original article in case you missed it…
Which do you do?…
Microbiologists are a very opinionated group of individuals, and it is, at times, difficult to get consensus and standardization on certain procedures. Whether to heat fix or methanol fix slides in preparation for Gram staining is one of those controversial procedures.
It was never fully documented as to why one method would be superior over the other…
Jeanne Minnerath et. al. at the Biology Department at St. Mary’s University of Minnesota decided to settle the matter. They examined heat and methanol fixed slides for two parameters; adherence of the bacterial cells to the slide and the ability of the gram positive bacteria not to become over decolorized.
showed that methanol fixation won by a long shot! There were 2.5 times as many Staphylococcus aureus
cells present on the slide with methanol fixation compared to heat fixation. This number increased to a factor of 10 when E. coli
was tested. The methanol fixation was superior in causing the specimen to adhere securely to the slide.
In addition, they found that Gram positive bacteria were far less likely to become over-decolorized with methanol rather than heat fixation. This confirmed the findings of Mangels, et al in 1984.
Heat fixation tends to damage and distort the delicate cell wall structures of bacteria. This distortion also happens with tissue and blood cells which can create background debris that may be confusing or misleading.
Some have a concern for the toxicity and flammability of methanol in the lab. However, now that fume hoods are usually available and Bunsen burners are mostly used for museum displays, this should not be a problem. So why do some microbiologists insist on heat fixation?
Curiously, Minnerath mentions in her study that of the 15 microbiology manuals that they examined, all of them recommended heat fixation. Only two of them even mentioned methanol as an alternative.
What is your opinion? Send
in your comments and MicroBytes will publish some of them in the next edition in October.
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All formats available…
Hardy offers a complete selection of microorganisms for your QC program. These MBL organisms are licensed derivatives from ATCC. They are available in the swab or pellet format; qualitative or quantitative. Order from Hardy for immediate service and delivery.
the entire microorganism catalog online.
The MBL EZ-AccuShot offers quantified bacteria for your Growth Promotion Studies.
Try out Part III of the numbers game…
You should be good at this by now!
Think about it…
* In winter, why do we try to keep the house as warm as it was in summer when we complained about the heat?
* How come you never hear father-in-law jokes?
* Why do people keep running over a string a dozen times with their vacuum cleaner, then reach down, pick it up, examine it, then put it down to give the vacuum one more chance?
* Why is it that no plastic bag will open from the end on your first try?
*How do those dead bugs get into those enclosed light fixtures?
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Wisdom to Ponder…
1894 ~ 1962
English computer scientist, mathematician, logician, and cyptographer. He is credited with breaking the secret Nazi “Enigma” code during WWII. His discovery was thought to shorten the war by about two years, thus saving millions of lives. He is known as the father of theoretical computer science and artificial intelligence.
“Sometimes it is the people no one can imagine anything of, who do the things no one can imagine.”
“We can only see a short distance ahead, but we can see plenty there that needs to be done.”
“Those who can imagine anything can create the impossible.”
“A computer would deserve to be called intelligent if it could deceive a human into believing that it was human.”
“We are not interested in the fact that the brain has the consistency of cold porridge.”
“No, I’m not interested in developing a powerful brain. All I’m after is just a mediocre brain, something like the President of the American Telephone and Telegraph Company.”
“Machines take me by surprise with great frequency.”
A working replica of Turing’s machine, known as the “bombe,” that broke the German “Enigma” code.
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Did you know?
- Is celebrating its 36th year of serving microbiologists.
- Manufactures from three ISO certified factories; one in California, Ohio, and Texas.
- Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
- Services over 10,000 labs and maintains a worldwide network of over 65 distributors.
Want to receive the MicroBytes
Newsletter at home?
Want to view past issues
a new chromogenic medium….
Hardy Diagnostics is pleased to inform our customers that HardyCHROM ESBL (Cat No. G321) has recently received clearance from the FDA.
ESBL E. coli on the new HardyCHROM ESBL Agar plate.
ESBL Klebsiella pneumoniae on HardyCHROM ESBL.
HardyCHROM ESBL serves as a selective and differential chromogenic medium containing a broad-spectrum beta-lactam intended for the qualitative and presumptive detection of K. pneumoniae
, K. oxytoca
, and E. coli
that produce an extended-spectrum beta-lactamase (ESBL). In addition, it tests for Enterobacteriaceae that are potentially non-susceptible to broad-spectrum cephalosporins.
This chromogenic medium is to be used with fecal specimens and is intended as an aid in the detection, identification of colonization, and control of these bacteria in a healthcare setting.
Subculture to non-selective media is required for confirming identification, antimicrobial susceptibility testing, and epidemiological typing.
Hardy is the only media manufacturer to offer a selective medium to detect ESBLs. To try a sample of HardyCHROM ESBL in your lab, inquire about HardyCHROM ESBL with your Sales Rep today!
of Hardy’s Chromogenic Media.
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Three heads are better than one!
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Online Ordering Made Easy!
a short video
to learn how easy it is
to order from Hardy on-line!
. Click. You’re Done!
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Watch resistance happen…
Watch a time lapse video from Harvard Medical School that shows how bacteria can develop resistance with alarming speed to antimicrobials due to mutations!
Good sense or nonsense?
Like many scientific principles introduced to popular culture, the idea of probiotics has become chaotic and confusing. Most of us know that probiotics are the “good” bacteria that help keep the digestive system healthy, but what are
prebiotics? A quick Google search will tell you that prebiotics are everything from chicory root, Jerusalem artichokes, under-ripe bananas, to my personal favorite, chocolate. It is basically the same thing nutritionists have been telling us for years: high fiber greens are good for you.
So what exactly is the definition of a prebiotic??
The concept of prebiotics was first introduced by Marcel Roberfroid and Glenn Gibson in 1995 where they defined prebiotics as “a non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon and, thus, improves host health.”1 Roberfroid’s paper only lists two prebiotics that fit this definition, trans-galactooligosaccharides and inulin. Since then, the definition of prebiotics has been revised and broadened. Currently, specific compounds and substances are being researched and listed as prebiotics. These compounds include the fructans, oligofructose and inulin, FOS, lactulose, as well as the galactan, galactooligosaccharide, resistant starch, pectin and other fiber components, and milk oligosaccharides.
Which organisms feast on prebiotics?
Current research suggests that
Bifidobacterium are the healthy target organisms, although there is also evidence to suggest we may want to research other target organisms for optimum health (e.g. the vegan vs. meat diet gut microbiome and their effects on health). In addition,
Faecalibacterium prausnitzii and
Akkermansia muciniphila have been shown to respond to specific prebiotics, indicating that different prebiotics may select for different target organisms. A study by Bird suggests we should consider prebiotics as “undigested dietary carbohydrates that are fermented by colonic bacteria yielding short chain fatty acids as end products,” further broadening the definition.2
Which foods contain prebiotics?
The discussion on exactly what constitutes a prebiotic continues, but generally most scientists agree on at least some variation of Roberfroid’s original definition. So what foods actually fit into this category? Surprise surprise, it’s high fiber green things! It seems we just can’t escape them. However, while most prebiotics are fiber, not all fiber is a prebiotic. To be beneficial, prebiotics must resist gastric acidity, be fermented by intestinal microflora, and provide some sort of support/stimulation for intestinal bacteria potentially associated with health and well-being.
Foods high in prebiotics include leeks, asparagus, chicory root, Jerusalem artichokes, garlic, onions, dandelion greens, wheat bran, and legumes. Chicory may be the best since it is 65% fiber. Sadly, chocolate is not on this list, although you can always use the antioxidants in dark chocolate as an excuse in a pinch.
So why do we care?
In 2010, Roberfroid published another paper summarizing the potential health benefits of prebiotics in conjunction with information we now know about the link between the gut microbiome and health. He claims that increases in
Bifidobacterium are a marker of good intestinal health, and their presence reduces symptoms associated with inflammatory bowel disease and irritable bowel syndrome. In infants, increases in the fecal concentration of
Bifidobacterium correlates with improved stool quality, which Roberfroid defined as pH, SCFA, frequency and consistency. The presence of this microorganism in higher concentrations is also believed to reduce an infant’s risk of gastroenteritis, infections, allergic symptoms, and eczema. He cites studies that have reported a reduction in the incidence of tumors and cancers, an increase in postmenopausal women’s calcium absorption, and even an effect on weight loss.
As much as we try to reason it’s OK to eat more mac and cheese, dietary health really does seem to come back to those leafy fibrous greens.
By Kate Massey
Technical Support Specialist
1. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics.
Gibson GR, Roberfroid MB
J Nutr. 1995 Jun; 125(6):1401-12.
2. Resistant starch, large bowel fermentation and a broader perspective of prebiotics and probiotics.
Bird AR, Conlon MA, Christophersen CT, Topping DL
Benef Microbes. 2010 Nov; 1(4):423-31.
3. Hutkins RW, Krumbeck JA, Bindels LB, Cani PD, Fahey G Jr., Goh YJ, Hamaker B, Martens EC, Mills DA, Rastal RA, Vaughan E, Sanders ME (2016). “Prebiotics: why definitions matter”. Curr Opin Biotechnol. 37: 1-7. doi:10.1016/j.copbio.2015.09.001. PMC 4744122 . PMID 26431716.
4. British Journal of Nutrition, Volume 104, Issue S2
August 2010, pp. S1-S63
Prebiotic effects: metabolic and health benefits
Marcel Roberfroid et all
Published online: 01 August 2010
For your plate counts…
Hardy now offers a better alternative when it comes to performing plate counts. Not only can Compact Dry plates be stored at room temp, and approved by AOAC, but also the sample automatically diffuses into the media! Never mess with spreaders and films again!
“I have noticed that even people who claim
everything is predetermined and that
we can do nothing to change it
look before they cross the road.”
Stephen Hawking, physicist
for brighter, more distinct color reactions.
all chromogenic media
Submitted by Rebecca Rodriguez
* A prisoner’s favorite punctuation mark is the period because it marks the end of his sentence.
* So what if I don’t know what apocalypse means. It’s not the end of the world!
*The biggest knight at King Arthur’s round table was Sir Cumference.
*A man just assaulted me with milk, cream, and butter. How dairy.
“As Hardy Diagnostics
enters its 36th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
Jay Hardy, CLS, SM(NRCM)
“Be careful about reading health books.
You may die of a misprint.”
Mark Twain ~
Have you ever wondered what it was like at the first Thanksgiving in the year 1621 at Plymouth?