a culture of service…
© 2017, Hardy Diagnostics,
all rights reserved
of Group B Strep…
Carrot Broth One Step
- Improved…No tile addition is needed!
- Detects hemolytic Group B Strep from the initial broth culture
- Provides results in as little as sixteen hours
- Found to be 100% sensitive and up to100% specific in a recent study
* * *
Automate your Wright’s-Giesma stain!
Hardy’s HemaPRO is the world’s most consistent, versatile, and durable blood smear slide stainer. Find out why…
a brief video about how easy it is to set up the HemaPRO in your lab.
our automated Gram stain instrument, GramPRO 1.
contact me to discuss automated slide stainers.
This 85 page booklet contains descriptions of the 2,700 products that Hardy manufactures for the microbiologist.
the digital version.
an account rep contact me about a price quote.
* * *
There is only one state in the US that will experience the sun’s rays to be directly overhead…and that is Hawaii. This happens on only two days every year and can be evidenced by flagpoles casting no shadow whatsoever.
This event happens only in locations between the Tropic of Cancer (23.5 degrees north) and the Tropic of Capricorn (23.5 degrees south) due the earth being tilted on its axis as shown below.
The ancient Hawaiian
name for the event was “kau ka lā i ka lolo
” which literally translates as “the sun rests on the brains.”
* * * * *
What is Hardy all about?
a short video to find out…
In need of a cranial workout?
Think about it…
* Why do we say something “is out of whack”? What’s a whack?
* When you choke a smurf, what color does it turn?
* If they arrested the Energizer Bunny, would they charge it with battery?
* I believe five out of four people have trouble with fractions.
* How come you never hear about gruntled employees?
* * *
Wisdom to Ponder…
Vincent van Gogh
1853 ~ 1890
Famous impressionist Dutch painter who, during his tempestuous life, created almost 900 paintings but only sold one during his lifetime.
“Great things are done by a series of small things brought together.”
“I dream of painting, and then I paint my dream.”
“For my part, I know nothing with any certainty, but the sight of the stars makes me dream.”
“I put my heart and my soul into my work and have lost my mind in the process.”
“There may be a great fire in our hearts, yet no one ever comes to warm himself at it, and the passers-by see only a wisp of smoke.”
“The best way to know God is to love many things.”
“I am still far from being what I want to be, but with God’s help, I shall succeed.”
“I feel that there is nothing more truly artistic than to love people.”
“One must work and dare if one really wants to live.”
“Love always brings difficulties, that is true, but the good side of it is that it gives energy.”
The artist at 25 years of age,
two years before he took up painting.
* * *
Online Ordering Made Easy!
Watch a short video
to learn how easy it is
to order from Hardy on-line!
Pick. . . Click. . .
And your order is on its way!
* * *
Did you know?
- Is celebrating its 37th year of serving microbiologists.
- Manufactures from three ISO certified factories; one in California, Ohio, and Texas.
- Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
- Services over 10,000 labs and maintains a worldwide network of over 80 distributors.
Want to receive the MicroBytes
Newsletter at home?
Want to view past issues
“As Hardy Diagnostics enters its 38th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
For your ordering convenience!
Did you know that of the 2,700 products that Hardy makes, 700 of them are now available on Amazon.
Pharmacy Microbiology – Compliance with USP <797>
Media Fill Challenge Testing
Hardy offers a complete line of products for the pharmaceutical arena to comply with the new USP <797> guidelines.
* * * * *
* * * * *
Resistance to β-Lactam Antibiotics
New Solutions to an Old Problem
As we progress through the 21st Century, the looming threat of antimicrobial resistance and the urgent call for action have been expressed by the medical community worldwide. Bacterial resistance mechanisms are becoming increasingly more common, especially to broad spectrum beta (β)-lactam antibiotics which include penicllins, cephalosporins, monobactams, and carbapenems.
β-lactam resistant bacteria generally evade these antibiotics through two paths: restriction of entry of the antibiotic into the bacterial cell and secretion of β-lactamase enzymes, which neutralize the antibiotic outside of the cell.
FDA cleared pairings of a β-lactam antibiotic and a β-lactamase inhibitor have been widely used in clinical settings for years; however, these combinations are still susceptible to bacterial resistance and may not be viable therapies for future treatment.
Although the recent advancements of new β-lactamase inhibitors is exciting towards fighting antibiotic resistance, older β-lactamase inhibitors are being paired with newer antibiotics and have shown similar effectiveness.
The β-lactamase inhibitor, tazobactam, discovered in the early 1980s has recently been paired with the 5th generation cephalosporin, ceftolozane, and marketed as Zerbaxa® (Ceftolozane/Tazobactam). This new drug combination is cleared for use to treat complicated urinary tract infections (UTIs) and intro-abdominal infections.
Newly discovered β-lactamase inhibitors, such as avibactam and vaborbactam, are showing promising results when paired with conventional β-lactams in treating some of the most antimicrobial resistant strains. For instance, the relatively new FDA cleared β-lactam and β-lactamase inhibitor combinations such as Ceftazidime/Avibactam (AvyCaz®) and Meropenem/Vaborbactam (Vabormere®) have also been effective in treating complicated UTIs and complicated intra-abdominal infections (AvyCaz®). Most importantly, these combination drugs have been successful against many resistant strains harboring extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and carbapenemases.
In an article in the Journal of Molecular Microbiology (Calvopiña et al., 2017), researchers have found that although AvyCaz has been shown to be effective clinically, the avibactam does not protect the ceftazidime completely from a particularly resistant β-lactamase producing strain of Stenotrophomonas maltophilia. However, when AvyCaz was paired with aztreonam, a monobactam β-lactam, the inhibition was extremely effective against the S. maltophilia strain. These new β-lactamase inhibitors have not been widely tested and with all β-lactam antibiotics, so their potential effectiveness is still untapped.
Hopefully, this study will be a gateway to further research β -lactamase inhibition and provide new breakthroughs and a potential to reverse antibiotic resistance.
Hardy Diagnostics manufactures many antibiotic disks
including the new Ceftazidime/Avibactam (AvyCaz) AST HardyDisks™ for disk diffusion testing. See Cat. nos. Z9351
(pack of 1 cartridge) and Z9355
(pack of 5 cartridges). Meropenem/Vaborbactam (Vabomere) has recently been cleared for use by the FDA and will be available in AST HardyDisks™ in December 2017 (Cat. nos. Z9321 and Z9325).
Another recently launched antibiotic that shows promise is Delafloxacin. While it does not qualify in the β-lactam/β-lactamase category, it is also available in HardyDisk™ (Cat. nos. Z9301
2. Karina Calvopiña, Philip Hinchliffe, Jürgen Brem, Kate J. Heesom, Samar Johnson, Ricky Cain, Christopher T. Lohans, Colin W. G. Fishwick, Christopher J. Schofield, James Spencer, Matthew B. Avison. Structural/mechanistic insights into the efficacy of nonclassical β-lactamase inhibitors against extensively drug resistant Stenotrophomonas maltophilia clinical isolates. Molecular Microbiology, 2017; 106 (3):492.
Cameron Whipple and Andre Hsiung
Two heads are better than one!
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Testimonial from a Pharmaceutical Lab worker…
“The two heads of TRIO BAS DUO air sampler was one of the best investments during the last two years. The laboratory staff responsible of the bacteriological sampling is able to double the number of environmental microbial cycles per day. This means more efficiency and lower cost, together with the possibility to increase in the future the number of sampling in other areas of the premises.”
Increase your recovery rate for anaerobes!
Hardy’s AnaeroGRO is packaged with an oxygen scavenger and flushed with nitrogen gas to ensure anaerobic conditions, leading to better recovery of anaerobic bacteria.
anaerobic microbiology supplies.
* * * * *
Can Microbiome Have
an Influence on Breast Cancer?
October is National Breast Cancer Awareness month, and while breast cancer is the most prevalent cancer among women worldwide, more than half the women who develop this cancer have no known risk factors. The risk factors that are known are not always good predictors of developing breast cancer, including those who are genetically predisposed and those who are exposed to known environmental risk factors.
For this reason, breast cancer is one of the most publicized and researched types of cancer. Naturally, microbiologists researching breast cancer look to the microbiome for clues and potential cures or treatments. This is not unprecedented; the composition of an individual’s microbiome has been linked to many diseases, either as causes or potential contributing factors, such as autism, diabetes, and asthma.
The general line of thinking for the microbiologists in the breast cancer research community is that variation in the human microbiome correlates to breast cancer development by modifying systemic estrogen levels. The Cleveland Clinic hypothesizes
that not only does this relationship exist, but it is potentially a causal relationship, with the microbiome modifying the systemic estrogen levels to the point that “cancerous breast tissue is associated with a microbiomic profile distinct from that of benign breast tissue, and that microbiomes of more distant sites, the oral cavity and urinary tract, will reflect dysbiosis as well.”
They argue that this is also related to the link between breast cancer and high-fat, low-fiber diets. They claim that while a distinct microbiome has been identified within breast milk and tissue, few studies have characterized the differences in the microbiome within women with or without cancer. They identify this as a hole in the research, and attempt to begin to fill it.
To investigate this hypothesis, fifty-seven women with invasive breast cancer undergoing mastectomy and 21 healthy women undergoing cosmetic breast surgery were enrolled. The bacterial 16S rRNA gene was amplified from urine, oral rinse, and surgically collected breast tissue, sequenced, and processed through a QIIME-based bioinformatics pipeline. They found that cancer patient breast tissue microbiomes were clustered in a significantly different way from non-cancer patients, namely by decreased levels of Methylobacterium in cancer patients. Their report showed no significant difference in oral rinse samples, and that differences in urinary microbiomes were largely correlated with menopausal status, with postmenopausal women showing decreased levels of Lactobacillus.
Cancer patients had increased levels of Gram-positive organisms including Corynebacterium, Staphylococcus, Actinomyces, and Propionibacteriaceae. They feel this suggests that the local breast microbiota differ in patients with and without breast cancer, but is possibly not a systemic difference. Cancer patient urinary microbiomes were characterized by increased levels of Gram-positive organisms in this study, but should be studied further due to possible confounding variables.
Despite the lack of findings provided in this study, other studies of microbiomic analysis of both breast milk and tissue shows that the human breast harbors unique and diverse microbiota; one that is at least partially derived from translocation of gut microorganisms. In addition to modulating the immune system, these gut microbes are known to play a vital role in estrogen metabolism; so they are not convinced that the lack of systemic evidence implies their initial hypothesis was incorrect. They suggest further studies on the breast tissue microbiome’s role in local estrogen metabolism and breast carcinogenesis are warranted.
by Kate Masey
Technical Support Specialist
* What is the difference between a nicely dressed man on a tricycle and a poorly dressed man on a bicycle? A tire.
* I used to be a banker but I lost interest.
* My friend’s bakery burned down last night. Now his business is toast.
* When Peter Pan punches, they Neverland
* Yesterday I accidentally swallowed some food coloring. The doctor says I’m OK, but I feel like I’ve dyed a little inside.
# # #
There will always be someone who will say something unkind about you.
Live your life so that no one will believe it.
* Man who drive like hell bound to get there.
* Man who sit on tack get point!
* He who thinks only of number one must remember this number is next to nothing.
* Man who eat crackers in bed wake up feeling crummy
* Man who jump through screen door, strains himself.
* * *