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a culture of service…

October, 2018

 

© 2018, Hardy Diagnostics,

all rights reserved 

 

 
Plazomicin now available!
New Antibiotics
 
Disks Now Available from Hardy
 
  HardyDisks
  • Plazomicin, “Zemdri” (Z9331)
  • Ceftazidime/Avibactam, “Avycaz”  (Z9351, Z9355)
  • Ceftolozane/Tazobactam, “Zerbaxa” (Z9341, Z9345)
  • Delafloxicin, “Baxdela” (Z9301, Z9305)
  • Meropenem/Vaborbactam, “Vabomere” (Z9321, Z9325)

 

HardyDisks:
  • Are compatible with all BD Disk dispensers.
  • Feature “last disk recognition” so you know when a refill is needed.
  • Include all traditional and newer antibiotics.
Learn more about HardyDisks.
View our AST mini-catalog.
Order Plazomicin.

 

 

For the detection

 

 of Group B Strep…
 
Carrot Broth One-Step
  • Improved…No tile addition needed!
  • Detects hemolytic Group B Strep from the initial broth culture
  • Provides results in as little as sixteen hours
  • Found to be 100% sensitive and up to 100% specific in a recent study
 
 
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Gram Staining made easy…
 

No Mess!
No Stress!
No Inconsistencies!
Hardy’s GramPRO is the world’s most consistent, repeatable, and reliable way to perform a Gram stain. Find out why…

 

 

Watch a brief video about how easy it is to set up the GramPRO in your lab.
Learn more about the GramPRO 1.

 

 

Please contact me to discuss automated slide stainers.

 

 

 

Dandruff

 
Is it fungal? bacterial?  or ???
It was long postulated that dandruff was attributed to a person’s individual susceptibility, hyperactive sebaceous glands, poor hygiene, the fungal genus Malassezia, or a combination thereof. Dandruff is an omnipresent condition that affects approximately 50% of the world’s population. It can vary in severity based on a multitude of factors, including, but not limited to: age, gender, host physiology, and host demographics to name a few.
For quite some time, dandruff studies were focused on the fungal genus Malassezia-the predominant species of fungi in the cutaneous biome-as they were proposed to be the causative agent of dandruff. As a result, most prescription-strength shampoos used in the treatment of dandruff contain a fungicidal compound called Zinc Pyrithione (ZPT), which acts to inhibit fungal growth through a mechanism of copper toxicity; however, most medicated shampoos and conditioners are ineffective in combating dandruff, which begs the question: “Is dandruff truly caused by a fungus?”
Recent efforts made by Zhijue Xu and his colleagues of Shanghai Jiao Tong University in China have elucidated that the presence of dandruff may be more closely associated to a commune of bacterial species than the once prospective agents, M. restricta and M. globosa, the most clinically significant species of the Malassezia genus. In their studies, published in May of 2016, a total of 363 adults with varying degrees of dandruff were sampled for an analysis of their cutaneous microflora. These fungal and bacterial profiles were compiled using pyrosequencing, which is a method of DNA sequencing that captures the generation of light when a pyrophosphate is released following deoxynucleotide phosphate (dNTP) incorporation by a polymerase.
The results of their findings revealed an alarming degree of similarity in the Malassezia spp. population between subjects with and without dandruff, suggesting that these organisms may not be the causative agent of dandruff after all. Interestingly enough, the results of the study revealed a correlation between dandruff and the composition of the bacterial commune on an individual’s scalp. Comprising this bacterial make-up are most notably Staphylococcus bacteria and Propionibacterium acnes, which both significantly vary depending on whether the subject had dandruff or not.
Additionally, it was discovered that there was a close association between the bacterial population and both water and sebum content on a person’s scalp. Samples from those with dandruff exhibited elevated levels of Staphylococcus bacteria and very low levels of Propionibacterium acnes, while also having dramatically lower water and sebum content on their scalp. These two microbes appear to be in direct competition with one other as the prevailing species appears to be linked with the absence or presence of dandruff. Propionibacterium acnes, a facultative anaerobe-releases free fatty acids through the hydrolysis of triglycerides (sebum) to promote bacterial adherence in addition to producing bacteriocidins to eliminate other species and thus competition. Individuals without dandruff showed the opposite: elevated levels of Propionibacterium acnes and very low levels of Staphylococcus, which also corresponds with greater water and sebum content in their scalps. If Staph is the main cause of the problem, many proposed natural means of treatment may be the best, such as vinegar with olive oil, tea tree oil, lemon juice, or baking soda. 
These findings, while revolutionary, also raises other questions: “Did the lack of water and sebum content give rise to a prevailing Staphylococcus population, or did the Staph cause the dry scalp in the first place?” While difficult to answer, the elucidation of these findings now gives reason to discontinue the use of medicated shampoos and look into alternative holistic treatments, which may better treat the condition while also avoiding the unwanted side effects of such shampoos.
References 12

 

 

by Andrew Kravariotis
R&D Technologist

 

HARDY DIAGNOSTICS

 

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Strange ads from the past…
 
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Phraseology…

“Straight from the Horses Mouth”
Everyone who follow horse races is always looking for an inside tip to give them an edge in picking the winner. 
 
The tip could come from the jockey, the stable boy, the trainer; but best yet would be “straight from the horses mouth.
 
First seen in a British newspaper in 1913:
 
“I got a tip yesterday, and if it wasn’t straight from the horse’s mouth it was jolly well the next thing to it.”
 
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What is Hardy all about?
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Hardy Goes Green…

green business cert

Once again, Hardy Diagnostics became re-certified as a Green Business in Santa Barbara County.  This documents the company’s efforts in reducing it’s energy needs, eliminating waste, encouraging recycling, and the installation of solar panels and LED lighting.

Hardy green

Think about it…
    
The Thinker

  

  • Why is it that when you transport something by car, it’s called a shipment, but when you transport something by ship it’s called cargo?
  • You know that little indestructible black box that is used on  planes? Why can’t they make the whole  plane out of the same substance?
  • Why is it that when you are driving and looking for an address, you turn the radio down? 
  • Why don’t sheep shrink when it rains?
  • Why are they called apartments when they are all stuck together?

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Wisdom to Ponder…

 
 
G. K. Chesterton
1874 ~ 1936
An English writer, poet, philosopher, dramatist, journalist, orator, lay theologian, biographer, and literary and art critic. Chesterton is often referred to as the “prince of paradox.

 

 

 

 

 

 

“I am not absentminded. It is the presence of mind that makes me unaware of everything else.”
“The true soldier fights not because he hates what is in front of him, but because he loves what is behind him.”
“Without education, we are in a horrible and deadly danger of taking educated people seriously.”
“The Bible tells us to love our neighbors, and also to love our enemies; probably because generally they are the same people.”
“There are two ways to get enough. One is to continue to accumulate more and more. The other is to desire less.”
“Do not be so open-minded that your brains fall out.”
“We do not need to get good laws to restrain bad people. We need to get good people to restrain us from bad laws.”
“Lying in bed would be an altogether supreme experience if one only had a colored pencil long enough to draw on the ceiling.”
“If men will not be governed by the Ten Commandments, they shall be governed by the ten thousand commandments”
“Education is simply the soul of a society as it passes from one generation to another.”

 

 

 

 

 

 

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Did you know? 
  Lauren
Hardy Diagnostics…
  • Is celebrating its 38th year of serving microbiologists.
  • Manufactures from two ISO certified factories; one in California and one in Ohio.
  • Is ISO 13485 certified for the manufacture of medical devices to give you confidence in our products.
  • Services over 10,000 labs and maintains a worldwide network of over 80 distributors.
  • Is a 100% Employee-Owned company. “If we act like we own the place…it’s because we do!”

 
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“As Hardy Diagnostics enters its 38th year of serving microbiologists in the laboratory, I would like to thank each of our customers for their support and loyalty. It truly has been a pleasure to serve you!
If there is any way we can improve or expand upon our service, would you please let me know?”
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Micro Musings…
 

Did you know that Hardy was the first company to introduce Chromogenic media to America in 1996? Hardy has been the leader in the field of easy identification by color ever since.
HC MRSA 48 hours
HardyCHROM MRSA, Cat no. G307

 

 

See our complete Chromogenic product offering.
View the online catalog.
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Stool cultures with no 
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HardyCHROM SS NoPRO

 

NOTE: New study shows 50% less in colony work-ups, for an overall cost saving of 80%!
Find out how you  can save also…
  • Reduces costly false-positive work-ups, due to Proteus spp.!
  • Less colony picking, subculturing, and identifications
  • No need for TSI, LIA, or KIA tubes
  • Reduces use of expensive ID cards
  • Reduces the number of plates for primary stool setup
  • Increased specificity
  • Easy Identification by patented chromogenic reaction
  • The only chromogenic media that will detect both Salmonella and Shigella
 
 
 
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Trio Bas
Air Samplers
 
Two heads are better than one!
 
Now with Bluetooth capability!
Trio Bas from Orum International has a robust impact air sampler for every type of use. Single, double, or triple heads are available from Hardy Diagnostics.
Testimonial from a Pharmaceutical Lab worker…
 
The two heads of TRIO BAS DUO air sampler was one of the best investments during the last two years. The laboratory staff responsible of the bacteriological sampling is able to double the number of environmental microbial cycles per day. This means more efficiency and lower cost, together with the  possibility to increase in the future the number of sampling in other areas of the premises.”
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For the reliable recovery of anaerobes…
 
 
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anaeroGRO
Learn more about anaerobic media.
View the anaerobic bacteriology catalog.
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The good organisms thrive with KD
Can Ketogenic Diets Improve Gut Health?

 

The ketogenic diet (KD) has increased in popularity among dieters over the past few decades and remains to be one of the more controversial diets due to its strict requirements. The diet has been used since the 1920s as a therapy for refractory epilepsy and more recently for Glucose Transporter 1 Deficiency Syndrome (GLUT1 DS). 
The diet is also under investigation for treating insulin resistance. Initially, KD was used to mimic the metabolism of fasting and was found to have a suppressant effect on epileptic seizures. For two decades, KD was used as a treatment for epilepsy prior to anti-epileptic drug treatments and is still used when patients are unresponsive to those medications.
Due to some of its beneficial effects on weight loss and metabolism, there has also been a major increase in research on KD over the last 15 years, and some of the more intriguing results include its effect on the microbiome. A study published in April 2018 by David Ma et al., in the Scientific Reports Journal showed that a ketogenic diet alters gut microbial diversity and increases pro-vascular microbiota. Microbial diversity, using the Shannon index for alpha diversity which is a measure of within-sample diversity and a synthesis of both the richness and evenness of the microbial community in a sample, was significantly lower in fecal samples from controls relative to the KD mice. It was found that there was an increase in potentially beneficial microorganisms and a decrease in potentially detrimental microorganisms. According to their data, the diet increased the relative abundance of beneficial gut microbiota (Akkermansia muciniphila and Lactobacillus), and reduced that of pro-inflammatory taxa (Desulfovibrio and Turicibacter) (1). Desulfovibrio are sulfate-reducing bacteria associated with inflammatory bowel disease. However, there are conflicting studies such as one conducted in 2017 by Tagliabue, A. et al., that showed a significant increase in Desulfovibrio spp. in patients affected by GLUT1 DS and subjected to KD(2). Due to conflicting results such as these, the subject continues to be studied, but evidence is mounting that organisms such as Desulfovibrio are reduced by the ketogenic diet.
The human body normally relies on carbohydrates for most of its quick energy, but when carbohydrates are reduced to a very low level, glycogen stores are reduced as levels of the hormone insulin decline. This allows fatty acids to be released from fat stores in the body and liver converts some of these fatty acids into the ketone bodies acetone, acetoacetate and beta-hydroxybutyrate, which can be used as fuel by parts of the brain. Due to the strict nutritional requirements to sustain KD (high fat, moderate protein, and low carbohydrates), the growth of some organisms in the gut are favored over the growth of others, especially those that do not ferment carbohydrates and are better adapted to a high fat and low carbohydrate environment. The diversity of the microbiome is therefore decreased and counter-intuitively may lead to some positive effects on health, such as a reduction in inflammation in the gut. Akkermansia muciniphila abundance is inversely associated with obesity and diabetes and is known to have an effect on fat storage, adipose tissue inflammation, and glucose metabolism. KD has been shown to increase potentially beneficial gut microorganisms, including A. muciniphila and bacteria from the genus Lactobacillus. Lactobacillus has been shown in several studies to decrease body weight and fat. The drug Metformin which is prescribed for type 2 diabetics to increase insulin sensitivity has also been reported to increase the abundance of A. muciniphila (3). This shows that lower glucose levels (which are observed in KD) in the body favor the growth of organisms such as A. muciniphila, which further reinforce its beneficial effects.
 
While there is still much to be learned on the subject, the ketogenic diet may be a practical solution for treating inflammation in the body, reducing body fat, and treating type 2 diabetes. Due to the strict requirements of the diet, remaining committed to its prescribed rules for longer periods of time may be somewhat unsustainable for most individuals. However, it could be a useful tool beyond treating epilepsy that can benefit health and guide users in helping them to understand how to make better food choices.
Ultimately, reducing the consumption of refined sugars, which are in very high amounts and ubiquitous in today’s processed foods, has been shown to significantly benefit human health. The reduction of detrimental microbiota and encouraging the growth of beneficial organisms in the gut may be an added benefit to the diet which also reinforces its overall utility.

 

References: 1, 2, 3

 

by Marcus Zuzow
R&D Microbiologist
HARDY DIAGNOSTICS

 

 
Xofluza Released
in Time for Flu Season
 The FDA recently cleared Genentech’s Xofluza for those experiencing influenza symptoms for less than 48 hours. This is the first new anti-viral drug to be approved in 20 years. Xofluza inhibits the replication of the flu virus and requires the oral ingestion of only a single tablet.The new drug significantly reduced the duration of flu symptoms by more than one day (median time 54 hours versus 80 hours).

 

 

 

   Optical oddities…
 

Everyone sees the three girls,
but can you find the three boys?

 

  “Believe half of what you see

and none of what you hear.”

 ~ Benjamin Franklin ~

 

RUBES

Find more

Want to book Leigh as a speaker at your next event?

Teenagers:
Respect Your Parents….
They made it through school without Google!
  * * *

Warning:
PUN ZONE
AHEAD
 

 

* When you get a bladder infection, urine trouble.
* Broken pencils are pointless.
* What do you call a dinosaur with an extensive vocabulary? A thesaurus.
* I dropped out of communism class because of lousy Marx.

 

* Man who runs in front of car gets tired, but man who runs behind car gets exhausted.

 

 

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Over 250 formulas that are used
by thousands of labs daily.

Take advice from the ocean…
    • Be shore of yourself
    • Come out of your shell
    • Take time to coast
    • Avoid pier pressure
    • Sea life’s beauty
    • Don’t get tide down
    • Make waves!

Learning English is easy… 
 
Anyone can learn it
through tough thorough thought, though.

 

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