In 1977, MacDade et al. were the first to isolate the bacterium, Legionella pneumophila, responsible for causing Legionnaire’s Disease. Following this discovery, there were numerous reports of Legionella being isolated from fresh water environments such as water distribution systems, evaporative condensers, air conditioning units, cooling towers, fountains, humidifiers, dentistry tools, shower heads, faucets and whirlpool spas or Jaccuzis®. To date, there are approximately 50 known species and 70 serogroups of Legionella that have been identified. In 1978, Feeley et al. developed a medium to provide consistent isolation of Legionella species. The medium demonstrated differential growth characteristics to aid in the identification of Legionella species.
Buffered Charcoal Yeast Extract Agar BCYE
BCYE (Buffered Charcoal Yeast Extract) Selective Agar with GPCV antibiotics
(Buffered Charcoal Yeast Extract Agar with Glycine, Vancomycin, Polymyxin, and Cycloheximide) For the selective isolation of Legionella spp. Conforms to ISO 11371 formulation. Hardy Diagnostics Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. W169 is based on Edelstein’s formula and is formulated in accordance with ISO 11371:(1998)E. This formulation employs the use of L-Cysteine, soluble ferric pyrophosphate, and alpha-ketoglutarate to enhance the growth of Legionella species. Activated charcoal removes toxic metabolic products. Protein and other growth nutrients are supplied by yeast extract.
BCYE Selective Agar (Buffered Charcoal Yeast Extract) with CCVC antibiotics
Buffered Charcoal Yeast Extract Selective Agar with Cephalothin, Colistin, Vancomycin, and Cycloheximide. For the selective isolation and cultivation of Legionella from environmental and clinical specimens.
BCYE Selective Agar (Buffered Charcoal Yeast Extract) with CAV antibiotics
Buffered Charcoal Yeast Extract Agar with Colistin, Anisomycin, and Vancomycin antimicrobials. For the selective isolation and cultivation of Legionella from environmental and clinical specimens.