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Buffered Charcoal Yeast Extract Agar BCYE

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In 1977, MacDade et al. were the first to isolate the bacterium, Legionella pneumophila, responsible for causing Legionnaire’s Disease. Following this discovery, there were numerous reports of Legionella being isolated from fresh water environments such as water distribution systems, evaporative condensers, air conditioning units, cooling towers, fountains, humidifiers, dentistry tools, shower heads, faucets and whirlpool spas or Jaccuzis®. To date, there are approximately 50 known species and 70 serogroups of Legionella that have been identified. In 1978, Feeley et al. developed a medium to provide consistent isolation of Legionella species. The medium demonstrated differential growth characteristics to aid in the identification of Legionella species.

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BCYE (Buffered Charcoal Yeast Extract) Selective Agar with GPCV antibiotics

(Buffered Charcoal Yeast Extract Agar with Glycine, Vancomycin, Polymyxin, and Cycloheximide) For the selective isolation of Legionella spp. Conforms to ISO 11371 formulation. Hardy Diagnostics Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. W169 is based on Edelstein’s formula and is formulated in accordance with ISO 11371:(1998)E. This formulation employs the use of L-Cysteine, soluble ferric pyrophosphate, and alpha-ketoglutarate to enhance the growth of Legionella species. Activated charcoal removes toxic metabolic products. Protein and other growth nutrients are supplied by yeast extract.

Cat. no. W169, 10/pk

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BCYE Page G07

BCYE Agar (Buffered Charcoal
Yeast Extract)

Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and isolation of Legionella spp. in water and other samples suspected of harboring the bacteria.

Cat. no. G07, 10/pk

BCYE Selective Agar (Buffered Charcoal Yeast Extract) with CCVC antibiotics

Buffered Charcoal Yeast Extract Selective Agar with Cephalothin, Colistin, Vancomycin, and Cycloheximide. For the selective isolation and cultivation of Legionella from environmental and clinical specimens.

Cat. no. G170, 10/pk

BCYE Selective Agar (Buffered Charcoal Yeast Extract) with CAV antibiotics

Buffered Charcoal Yeast Extract Agar with Colistin, Anisomycin, and Vancomycin antimicrobials. For the selective isolation and cultivation of Legionella from environmental and clinical specimens.

Cat. no. G08, 10/pk

SASKitLegionella

SAS™ Legionella Rapid Test

A visual read out, in vitro immunochromatographic rapid assay for the presumptive qualitative detection of Legionella pneumophila serogroup 1 antigens in human urine. This test is intended to aid in the presumptive diagnosis of Legionnaires’ disease in conjunction with culture and other methods for patients with signs and symptoms of pneumonia.

Cat. no. 044020, 20 test per box

SAS kit

Legionella Controls, for SAS™ Legionella Test Kit

SAS™ Legionella Controls are for use in monitoring the in vitro diagnostic performance of the SAS™ Legionella Test (Cat. no. 044020) presumptive qualitative detection of Legionella pneumophila serogroup 1 antigens in human urine.

Cat. no. 044413

Legionella Agglutination Test

Legionella Latex Kit Latex Agglutination Test

A latex agglutination test for the presumptive identification of predominant Legionella spp. cultured from clinical or environmental sources, takes less than one minute to complete, easy to read blue latex, tests for Legionella pneumophila serogroup 1, 2 through 14, and seven other pathogenic Legionella spp., sensitivity is 98.8%, specificity is 100%, kit includes three latex reagents, controls, buffer, and cards.

Cat. no. DR800M, 50 tests per box

LegionellaDirectFlourescentTest

Legionella Direct Fluorescent Monoclonal Antibody Test Kit

Pro-Lab Diagnostics offers a full range of FITC-labeled monoclonal & polyclonal antibody kits and single reagents for the detection and identification of Legionella pneumophila serogroups 1-14 as well as Legionella micdadei. The kit is supplied complete with L. pneumophila serogroup 1 DFA reagent, positive and negative controls, and mounting medium, sufficient for 50 tests.

Cat. no. PL241, 50 tests per box