Hardy Diagnostics CRITERION™ HardyCHROM™ Vibrio is recommended for use as a selective and differential growth medium for the cultivation, isolation, and differentiation of�Vibrio�spp. from food and environmental samples.

The�Vibrio�genus is comprised of Gram-negative, halophilic, non-spore forming rods that are straight or have a single, rigid curve. All�Vibrio�spp. are motile, and most species are oxidase- and catalase-positive. Several species in the genus are known human pathogens, the most notable of which are�V. cholerae,�V. parahaemolyticus, and�V. vulnificus. Most disease-causing strains are associated with gastroenteritis following ingestion of raw or uncooked shellfish or drinking contaminated water. Cases of open wound infection and septicemia have also been reported.

V. cholerae is the most well known�Vibrio�species, as it is the causative agent of cholera. This organism is most often transmitted via contaminated drinking water in developing countries. V. parahaemolyticus is the leading cause of bacterial diarrhea associated with seafood consumption. It is transmitted through ingestion of undercooked or mishandled seafood, or less commonly through open wounds exposed to seawater. While not as common, V. vulnificus is the most fatal�Vibrio�species in the United States. Infection and death from this species occurs from consumption of Gulf Coast oysters and possibly wound infections acquired in or exposed to marine environments.

CRITERION™ HardyCHROM™ Vibrio Agar is a chromogenic medium, highly selective for isolation of�V. parahaemolyticus,�V. vulnificus,�V. cholerae�and�V. alginolyticus, as well as other�Vibrio�species. HardyCHROM™ Vibrio Agar has a high pH, which suppresses the growth of non-Vibrio�species found in similar marine samples. The media consists of animal proteins, sea salts, sodium citrate, sodium thiosulfate, sugars, and a chromogenic mix. Seas salts are incorporated into the medium to provide optimum growth and metabolic activity of halophilic�Vibrio�spp. Sodium thiosulfate provides a source of sulfur. The inclusion of chromogenic substrates allows for the differentiation of�Vibrio�species based on colony color. It is the only medium that will differentiate�V. vulnificus�from�V. parahaemolyticus�and�V. cholerae.

CRITERION™ Dehydrated Culture Media by Hardy Diagnostics is formulated to meet or exceed the highest standards. Compare these features:

• Wide mouth opening - reduces dust formation by allowing the easy entry of a scoop
• Convenient handgrip - 'no slip' finger holds
• Stackable containers - for efficient storage
• Increased solubility - dissolves quickly
• Custom and bulk products available - to your specifications
• Competitive pricing - call for a price quote for large volume purchases!
• Complete technical/instruction sheets
• Unparalleled performance - tested for optimal growth

Container sizes include:

• 2L mylar® bag - pre-measured for a 2 liter batch, no weighing needed!
• 500gm bottle - wide mouth, easy grip bottle
• 2kg and 10kg bucket - with convenient screw lid

Also see our selection of petri plates, glass screw-cap tubes, animal blood, media supplements and antibiotics.