Scientific Posters
Hardy Diagnostics has a very proactive R&D department that is continually looking for a better way to culture and identify pathogenic organisms. We collaborate with numerous outside laboratories to thoroughly test our innovative new products. To view video demo of selected products click here .
Below we have listed some recent posters that have been presented at various national ASM conventions.
C. diff Banana Broth Comparison of broth media for recovery of Clostrioides Difficile from environmental Samples
HardyDisk AST Susceptibility Disks Performance of HardyDisk Ceftolozane-Tazobactam Compared to Reference Broth Microdilution for Enterobacteriaceae and Pseudomonas aeruginosa Isolates.
Presented at ASM 2018 – Atlanta, GA
AnaeroGRO™ Anaerobic Culture Media
A Comparison of Commercially Prepared Culture Media for the Isolation of Anaerobic Bacteria
Presented at ASA 2012 – San Fransisco, California
AnaeroGRO™ media demonstrated superior performance for all strains tested on Brucella, BBE, and LKV.
Evaluation of BluEcoli™:
A New Chromogenic Medium for the Isolation and Identification of Urinary Tract Pathogens
Presented at ASM 2005 – Atlanta, Georgia
In-house testing and clinical trials demonstrated that BluEcoli™ can accurately detect E. coli without having to do further confirmatory testing. BluEcoli™ demonstrated 100% sensitivity and 100% specificity in both evaluations.
A New Test System for Identification of Neisseria spp. and Moraxella catarrhalis
Presented at ASM 2008 – Boston, Massachusetts
Describes the evaluation of CarboFerm™ – a carbohydrate utilization test system for the identification of Neisseria and Moraxella spp . The test system performed well for the identification of N. gonorrhoeae , N. meningitidis and M. catarrhalis . Further testing may be required for N. lactamica and Neisseria spp.
Evaluation of Dye Tolerance Media for the Identification of Brucella Species
Presented at ASM 2007 – Toronto, Canada
The study confirmed the Dye Tolerance media as a reliable and convenient method for the speciation of Brucella spp .
EnteroScreen4™
A Single-Tube Screen for Salmonella and Shigella
An article describing the use of a single-tube reaction system for the differentiation of non-pathogenic and pathogenic Enterobacteriaceae.
HardyCHROM ESBL Evaluation
Evaluation of HardyCHROM ESBL as a detection method of ESBL-producing Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca: a multi-centric study.
Presented at ASM 2017- New Orleans, Louisiana
The study concludes that HardyCHROM ESBL can be reliably employed as an initial screen for patients harboring Enterobacteriaceae conferring resistance to extended spectrum beta lactamases and 3rd generation cephalosporins.
Comparison of Disk Diffusion (M44-A) Assay with Reference (M38-A Microdilution) Method for Testing Moulds Against Voriconazole
Presented at ISHAM 2006 – Paris, France
The study compared disk diffusion testing of voriconazole mold isolates using 1 and 10ug disks and two media, Mueller Hinton with Glucose and Methylene Blue and standard Mueller Hinton plates.
Evaluation of GBS Detect as a Subculture Medium from LIM Broth for the detection of Group B Streptococcus: a multi centric study
Presented at ASM 2017 – New Orleans, Louisiana
Results of this evaluation support the usage of more GBS specific products in lieu of subculture to blood agar.
Evaluation of Optimal Storage Temperature, Time and Transport Medium for the Detection of Group B Streptococcus in StrepB Carrot Broth
Presented at ASM 2012 – San Fransisco, CA
StrepB carrot broth is a chromogenic enrichment broth that can directly detect hemolytic GBS isolates.
Molecular vs. Culture: Detection of Group B Streptococcus (GBS) Colonization in Pregnancy
Presented at SCACM 2012 – Indianapolis, IN
Carrot broth reliably detects beta hemolytic GBS in 16-24 hours.
Evaluation of GBS Detect™and PNA FISH™ for the Detection of Group B Streptococci in Subcultures of LIM Broth
Presented at ASM 2011 – New Orleans, LA
Based on the results of this study, GBS Detect™ is a reliable method to increase the sensitivity of traditional culture methods for GBS detection, especially for non-beta hemolytic strains.
Evaluation of GBS Detect™: A New Medium for the Detection of Non-Hemolytic Group B Strep in Subcultures of Carrot Broth™ and LIM Broth.
Presented at ASM – Boston, Massachusetts
A new medium, GBS Detect™, was evaluated in a multi-center trial in comparison to Blood Agar plates. Over 22% of the GBS isolates were detected only on GBS Detect™, because these strains were non-hemolytic on standard Blood Agar plates.
Evaluation of GBS Detect™ and ChromID Strepto B agars for the detection of Group B streptococcus from GBS Screening Broth Medium (GBS).
Presented at ASM – San Fransisco, California
Hardy Diagnostics GBS Detect media is the most reliable selective media for the detection of GBS when using the CDC culture algorithm.
Comparison of Four Media for the Detection of Group A Streptococcus from Throat Specimens.
Presented at ASM – Boston, Massachusetts
Hardy’s GBS Detect agar detected the most (90%) isolates after 24 hours.
Evaluation of Granada Medium as a Subculture Method from LIM Broth for the Detection of Group B Streptococcus: A Multi-Centric Study.
Presented at ASM 2017 – New Orleans, Louisiana
Granada Medium demonstrated 96.7% sensitivity and 98.4% specificity compared to the reference method based on color reaction alone. Sensitivity of Granada Medium was enhanced to 99.2% upon latex confirmation of non-hemolytic Group B Strep strains (white colonies on Granada). Ten strains of beta hemolytic GBS were undetected by LIM-BAP while these were recovered by the LIM-Granada agar.
Comparison of HardyCHROM™ Candida with the Becton Dickinson CHROMagar™ Candida for the Identification of Commonly Isolated Yeast Pathogens
Presented at ASM 2010 – San Diego, California
HardyCHROM™ Candida performed better than BD CHROMagar™ with larger colony size and better color development.
Comparison of HardyCHROM™ Candida with the Vitek® Yeast Biochemical Card Identification System for the Identification of Common Yeast Pathogens
Presented at ASM 2008 – Boston, Massachusetts
HardyCHROM™ Candida was found to be an easy, reliable, and accurate method for identifying clinically significant yeasts.
Evaluation of Two Types of Chromogenic Media for the Detection of MRSA in Wound Swabs
Presented at ASM 2011 – New Orleans, LA
The accurate identification of MRSA positive wound swabs was found to be more reliable on the HardyCHROM™ MRSA media within 24 hours in comparison with BBL™ CHROMagar™ MRSA media.
Evaluation of HardyCHROM™ MRSA in Comparison with BBL CHROMagar™ MRSA for the Detection of MRSA in Nasal Swabs
Presented at ASM 2010 – San Diego, California
HardyCHROM™ MRSA was found to be more reliable and had a faster detection time when compared to BBL CHROMagar™ MRSA.
Evaluation of Two Chromogenic Media for MRSA Screening from Nasal Swabs
Presented at ASM 2012 – San Fransisco, California
Bio-Rad MRSASelect and HardyCHROM MRSA accurately detect MRSA isolates from nasal swab specimens. HardyCHROM MRSA performs reliably at 24 hours, and is actually more accurate than a 48 hour interpretation. In our laboratory, visual clarity of the Hardy media was much preferred compared to the Bio-Rad product.
Evaluation of a Novel Chromogenic Medium for the Isolation and Differentiation of Salmonella and Shigella spp.
Presented at ASM 2011 – New Orleans, LA
Based on these findings, HardyCHROM™ SS can be employed as a cost effective, reliable method for the selective isolation and differentiation of Salmonella and Shigella spp. from non-pathogenic enteric bacteria
Evaluation of a Novel Chromogenic Medium as a Replacement for MacConkey Agar and Hektoen Enteric Agar
Presented at ASM 2012 – San Fransisco, California
Based on these findings, HardyCHROM SS can be employed as a replacement for MacConkey Agar and Hektoen Enteric Agar, as well as TSI and LIA screens from these media. HardyCHROM SS is a reliable and economical method for the selective isolation and differentiation of Salmonella and Shigella spp. from non-pathogenic enteric bacteria.
Evaluation of HardyCHROM™ UTI for the Isolation and Identification of Common Urinary Tract Pathogens
Presented at ASM 2006 – Orlando, Florida
HardyCHROM™ UTI proved to be an accurate and reliable primary medium for the differentiation and identification of common UTI pathogens found in urine specimens.
MacConkey w/Cipro and BHI w/Cipro – Liss Formulation
Detection of Fluoroquinolone Resistant Organisms from Rectal Swabs
Presented at ASM 2010 – San Diego, CA
These media allow for reliable recovery of ciprofloxacin-resistant enteric gram-negative organisms that can be introduced into the blood stream during prostate biopsy procedures.
Variation in Colonial Morphology of Fungal Isolates Exhibited by Different Malt Extract Products in Agar Media
Presented at ASM 2006 – Orlando, Florida
Evaluation of a new formulation of Malt Extract Agar that resulted in more restricted colonies and better sporulation.
Middlebrook 7H11/7H11 Comparison
Compare Hardy Diagnostics Middlebrook 7H11/Middlebrook 7H11 Selective Biplate to the equivalent Becton Dickinson product
Research Summary Report February 2014
The average colony size on the selective side of the HDx plate was larger than the average colony size on the selective side of the BD plate.
Evaluation of Rapid Methyl-Alpha-D-Glucopyranoside Medium (MGP) for the Differentiation of Vancomycin Resistant Enterococci .
Presented at ASM 2005 – Atlanta, Georgia
Rapid MGP Medium is a reliable means to differentiate E. faecalis and E. faecium from E. gallinarum and E. casseliflavus.
“The most impressive results occurred with the Hardy Diagnostics Trehalose Fermentation Broth. The sensitivity was 96%, with a specificity of 100%.”
The sensitivity was 96%, with a specificity of 100%.
Cost Effective Use of Rapid Urease Medium in Diagnosing H. pylori Gastritis
Presented at ASM 1997 – Miami, Florida
Rapid Urease Medium is more cost effective and has a faster reaction time as compared to the standard CLO test.
Evaluation of StrepB Carrot Broth™ and LIM Broth for Recovery of Group B Streptococci
Presented at the Chinese Laboratory Medicine Conference 2011 – Taipei, Taiwan
StrepB Carrot Broth™ revealed the best performance among the three culture media in this evaluation.
Comparison of LIM Broth with PNA FISH to Carrot Broth with PNA FISH for Identification of Group B Streptococcus in Prenatal Vaginal/Rectal Specimens
Presented at ASM 2010 – San Diego, CA
Group B Streptococcus Detection by Carrot Broth™/CHROMagar™ Colorex® GBS Agar vs. Conventional Colistin/Nalidixic Acid Agar/Group B Strep Broth Method
Carrot Broth™ demonstrated higher sensitivity when compared to another GBS broth.
Validation of Strep B Carrot Broth™ Using A Prospective Double Swab Comparison Method
Presented at CACMID 2008
Compared to conventional testing for GBS, using Strep B Carrot Broth™ demonstrated increased sensitivity and specificity with a marked reduction in turnaround time.
Evaluation of StrepB Carrot Broth™ versus Lim Broth
for Detection of Group B Streptococcus Colonization Status of Near-Term Pregnant Women
Diagnostic efficiency was increased with the use of Carrot Broth™ over LIM broth, because of the color change seen with positive samples.
Comparison of Carrot Broth™- and Selective Todd-Hewitt Broth-Enhanced PCR Protocols
for Real-Time Detection of Streptococcus agalactiae in Prenatal Vaginal/Anorectal Specimens
Testing negative Carrot Broth™ by PCR resulted in a higher percentage of positives than when LIM was used for PCR testing.
Carrot broth-enhanced Real-time PCR as an Alternative Means for Rapid Detection of Streptococcus agalactiae
Carrot broth culture using the BD GeneOhm™ StrepB assay (carrot broth enhanced PCR) yields increased sensitivity over carrot broth culture for detection of Streptococcus agalactiae .
Comparison of Culture Methods for the Detection of Group B Streptococcus from Vaginorectal Specimens
Presented at CACMID
Optimal results were obtained when using Carrot Broth™ for processing specimens for Group B Streptococcus .
Evaluation of StrepB Carrot Broth™ and LIM Broth for Long Term Storage/Recovery of Group B Streptococcus
Presented at ASM 2006 – Orlando, Florida
A study was conducted to determine survival of Group B Streptococcus in Carrot Broth™ and LIM Broth. The survival ranged was between 15-113 days in Carrot Broth™, with a mean of 51 days. Overall, the recovery from Carrot Broth™ stored at room temperature was better than with LIM broth stored under the same conditions.
Evaluation of Three Commercial Broth Media for Pigment Detection and Identification of Group B Streptococcus ,
(Streptococcus agalactiae)
Carrot Broth™ was compared to two other commercial broth media with similar recovery noted for all three products, except when the count was less than 10 -3 Recovery was better with Carrot Broth™ and Granada Instant Broth Biphasic when the CFU was lower.
Comparison of Commercially Available Methods (StrepB Carrot Broth™ and GBS Medium) for the Detection of Group B Streptococcus
Presented at ASM 2006 – Orlando, Florida
Study showed that Carrot Broth™ performed better than Group B Strep Broth(NEL).
Evaluation of Accuracy of StrepB Carrot Broth™ in the Detection of Different Serotypes of Group B Streptococci (GBS)
Presented at ASM 2006 – Orlando, Florida
Reference strains were tested using Carrot Broth™ and showed 100% sensitivity and specificity for all hemolytic strains tested.
Comparison of Real-Time PCR (Cepheid SmartCycler®) with Standard LIM Broth Culture and StrepB Carrot Broth™ for the Detection of Group B Streptococcus in Pre-Natal Vaginal/Rectal Specimens
Presented at ASM 2006 – Orlando, Florida
This study showed that Carrot Broth™ was more sensitive than LIM Broth and also that Carrot Broth™ showed better sensitivity than primary testing with the IDI-Strep BTM SmartCycler®.
Evaluation of StrepB Carrot Broth™ and LIM Broth Methods for Recovery of Group B Streptococci (GBS).
Presented at ASM 2005 – Atlanta, Georgia
Results of a multi-center trial comparing LIM Broth to Carrot Broth™. Carrot Broth™ demonstrated higher sensitivity and specificity than the LIM Broth method.
Evaluation of Three Methods (StrepB Carrot Broth™, LIM Broth, and Granada Agar) for Recovery of Group B Streptococci
Presented at ASM 2004 – New Orleans, Louisiana
This study compared three media used for the isolation of GBS. In cases where the colony count was low, Carrot Broth™ was able to reliably detect GBS.
Evaluation of New Group B Strep Carrot Broth One-Step in the Detection of Group B Streptocococcus: a multi-centric study
Presented at ASM 2017 – New Orleans, Louisiana
This study demonstrates that Carrot Broth One Step is equivalent in performance to the original Carrot Broth Kit, and is superior in testing due to no tile requirements, thus reducing specimen processing time.
People at Hardy Diagnostics are courteous, friendly and helpful.
Christina Benson, QC Microbiologist
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