Amies media is Stuart's original media formula modified by replacing the glycerophosphate in the original formula for an inorganic phosphate buffer. The metabolism of glycerophosphate by coliform organisms and other Gram-negative rods, in Stuart's original formulation, resulted in proliferation of these organisms from throat, wound and fecal specimens. Amies also found that NaCl at 0.3% w/v was optimal for the preservation of Neisseria gonorrhoeae. Calcium and magnesium salts were added in the belief that these ions were of importance in controlling the permeability of the bacterial cells and contributed to their survival.

Hardy Diagnostics offers Amies Medium in two formats:
Liquid Amies for Aerobic Culture Amies Agar Gel (with and without charcoal) for Aerobic and Anaerobic Culture

Unique Tube Shape Preserves Gel Media
Unique Venturi hourglass tube design prevents disintegration or breakdown of gel column during specimen transport and eliminates harmful air bubbles.

Maximum Gel for Anaerobe Preservation
5mL gel column affords maximum protection and improves sample viability.

Available With or Without Charcoal
The presence of charcoal in the medium neutralizes toxins and inhibitory substances. Amies formula with charcoal has been shown to improve the recovery of Neisseria gonorrhoeae and is often recommended for Bordetella pertussis